Osteopontin | HUABIO ER1802-16 product information

product summary

company name :

HUABIO

product type :

antibody

product name :

Osteopontin

catalog :

ER1802-16

quantity :

100μl

price :

330 USD

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

reactivity :

human, mouse

application :

ELISA, immunohistochemistry - paraffin section

more info or order :

HUABIO product webpage

image

image 1 :

ICC staining Osteopontin in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

image 2 :

ICC staining Osteopontin in MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

image 3 :

Immunohistochemical analysis of paraffin-embedded human liver cancer tissue using anti-Osteopontin antibody. Counter stained with hematoxylin.

product information

SKU :

ER1802-16

Target name :

Osteopontin

Species reactivity :

Human,Mouse

Applications :

IF-Cell,IHC-P,ELISA

Conjugate :

Non-conjugated

Immunogen :

Synthetic peptide within mouse Osteopontin 17-100.

Uniprot id :

P10451>SwissProt: P10451 Human;SwissProt: P10923 Mouse

Host :

Rabbit

Isotype :

IgG

Size :

100μl

List Price :

330 USD

Storage Buffer :

1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.

Form :

Liquid

Storage Instruction :

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.

Purity :

Immunogen affinity purified.

Product type :

Rabbit polyclonal Antibody

Positive control :

HepG2, MCF-7, human liver cancer tissue, human kidney tissue, mouse kidney tissue.

Molecular wt :

Predicted band size: 35 kDa

Subcellular location :

Secreted.

Concentration :

1 mg/mL.

Recommended dilutions :

IF-Cell: 1:50-1:200 ;IHC-P: 1:50-1:200 ;ELISA: 1:5,000-1:10,000

Advanced Validation :

Cell treatment (CT)

Pic img4 :

https://storage.huabio.cn/huabio/productImg/ER1802-16_4.jpg

Pic legend4 :

Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-Osteopontin antibody. Counter stained with hematoxylin.

Pic img5 :

https://storage.huabio.cn/huabio/productImg/ER1802-16_5.jpg

Pic legend5 :

Immunohistochemical analysis of paraffin-embedded mouse kidney tissue using anti-Osteopontin antibody. Counter stained with hematoxylin.

Pic img6 :

https://storage.huabio.cn/huabio/productImg/ER1802-16_6.jpg

Pic legend6 :

Immunocytochemistry analysis of RAW264.7 cells treated with LPS (100 ng/ml 6h) add BFA (300 ng/ml 3h) labeling Osteopontin with Rabbit anti-Osteopontin antibody (ER1802-16) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-Osteopontin antibody (ER1802-16) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (M1305-2, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution.

more info or order :

HUABIO product webpage