LSD1 | HUABIO ER1802-12 product information

product summary

company name :

HUABIO

product type :

antibody

product name :

LSD1

catalog :

ER1802-12

quantity :

100μl

price :

330 USD

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

reactivity :

human, mouse, rat

application :

western blot, flow cytometry, immunohistochemistry - paraffin section

more info or order :

HUABIO product webpage

image

image 1 :

Western blot analysis of LSD1 on different lysates with Rabbit anti-LSD1 antibody (ER1802-12) at 1/1,000 dilution. Lane 1: PC-12 cell lysate Lane 2: SiHa cell lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 93 kDa Observed band size: 100/80 kDa Exposure time: 1 minute 30 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ER1802-12) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature.

image 2 :

Immunohistochemical analysis of paraffin-embedded rat kidney tissue using anti-LSD1 antibody. Counter stained with hematoxylin.

image 3 :

Immunohistochemical analysis of paraffin-embedded human colon cancer tissue using anti-LSD1 antibody. Counter stained with hematoxylin.

product information

SKU :

ER1802-12

Target name :

LSD1

Species reactivity :

Human,Mouse,Rat

Applications :

WB,IF-Cell,IHC-P,FC

Conjugate :

Non-conjugated

Immunogen :

Synthetic peptide within C-terminal human LSD1.

Uniprot id :

O60341>SwissProt: O60341 Human;SwissProt: Q6ZQ88 Mouse

Host :

Rabbit

Isotype :

IgG

Size :

100μl

List Price :

330 USD

Storage Buffer :

1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.

Form :

Liquid

Storage Instruction :

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.

Purity :

Immunogen affinity purified.

Product type :

Rabbit polyclonal Antibody

Positive control :

PC-12 cell lysates, SiHa cell lysates, A431, HepG2, SH-SY-5Y, rat kidney tissue, human colon cancer tissue, human spleen tissue, human kidney tissue, mouse cerebellum tissue, K562.

Molecular wt :

Predicted band size: 93 kDa

Subcellular location :

Nucleus.

Concentration :

1 mg/mL.

Recommended dilutions :

WB: 1:500-1,000 ;IF-Cell: 1:500-1:2,000 ;IHC-P: 1:50-1:200 ;FC: 1:50-1:100

Pic img4 :

https://storage.huabio.cn/huabio/productImg/ER1802-12_4.jpg

Pic legend4 :

Immunohistochemical analysis of paraffin-embedded human spleen tissue using anti-LSD1 antibody. Counter stained with hematoxylin.

Pic img5 :

https://storage.huabio.cn/huabio/productImg/ER1802-12_5.jpg

Pic legend5 :

Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-LSD1 antibody. Counter stained with hematoxylin.

Pic img6 :

https://storage.huabio.cn/huabio/productImg/ER1802-12_6.jpg

Pic legend6 :

Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue using anti-LSD1 antibody. Counter stained with hematoxylin.

Pic img7 :

https://storage.huabio.cn/huabio/productImg/ER1802-12_7.jpg

Pic legend7 :

ICC staining LSD1 in A431 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Pic img8 :

https://storage.huabio.cn/huabio/productImg/ER1802-12_8.jpg

Pic legend8 :

ICC staining LSD1 in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Pic img9 :

https://storage.huabio.cn/huabio/productImg/ER1802-12_9.jpg

Pic legend9 :

ICC staining LSD1 in SH-SY-5Y cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Pic img10 :

https://storage.huabio.cn/huabio/productImg/ER1802-12_10.jpg

Pic legend10 :

Flow cytometric analysis of K562 cells with LSD1 antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black).

more info or order :

HUABIO product webpage