Smad4 | HUABIO ER1706-90 product information

product summary

company name :

HUABIO

product type :

antibody

product name :

Smad4

catalog :

ER1706-90

quantity :

100μl

price :

330 USD

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

reactivity :

human, mouse, rat

application :

flow cytometry, immunohistochemistry - paraffin section

more info or order :

HUABIO product webpage

image

image 1 :

ICC staining Smad4 in A549 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

image 2 :

ICC staining Smad4 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

image 3 :

ICC staining Smad4 in SH-SY5Y cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

product information

SKU :

ER1706-90

Target name :

Smad4

Species reactivity :

Human,Mouse,Rat

Applications :

IF-Cell,IHC-P,FC

Conjugate :

Non-conjugated

Immunogen :

Synthetic peptide within human Smad4 aa 500-542.

Uniprot id :

Q13485>SwissProt: Q13485 Human;SwissProt: P97471 Mouse;SwissProt: O70437 Rat

Host :

Rabbit

Isotype :

IgG

Size :

100μl

List Price :

330 USD

Storage Buffer :

1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.

Form :

Liquid

Storage Instruction :

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.

Purity :

Immunogen affinity purified.

Product type :

Rabbit polyclonal Antibody

Positive control :

A549, Hela, SH-SY5Y, rat large intestine tissue, human placenta tissue, mouse small intestine tissue, HUVEC.

Molecular wt :

Predicted band size: 60 kDa

Subcellular location :

Cytoplasm, Nucleus.

Concentration :

1 mg/mL.

Recommended dilutions :

IF-Cell: 1:50-1:200;IHC-P: 1:50-1:100;FC: 1:50-1:100

Pic img4 :

https://storage.huabio.cn/huabio/productImg/ER1706-90_4.jpg

Pic legend4 :

Immunohistochemical analysis of paraffin-embedded rat large intestine tissue using anti-Smad4 antibody. Counter stained with hematoxylin.

Pic img5 :

https://storage.huabio.cn/huabio/productImg/ER1706-90_5.jpg

Pic legend5 :

Immunohistochemical analysis of paraffin-embedded human placenta tissue using anti-Smad4 antibody. Counter stained with hematoxylin.

Pic img6 :

https://storage.huabio.cn/huabio/productImg/ER1706-90_6.jpg

Pic legend6 :

Immunohistochemical analysis of paraffin-embedded mouse small intestine tissue using anti-Smad4 antibody. Counter stained with hematoxylin.

Pic img7 :

https://storage.huabio.cn/huabio/productImg/ER1706-90_7.jpg

Pic legend7 :

Flow cytometric analysis of HUVEC cells with Smad4 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.

more info or order :

HUABIO product webpage