iNOS | HUABIO ER1706-89 product information

product summary

company name :

HUABIO

product type :

antibody

product name :

iNOS

catalog :

ER1706-89

quantity :

100μl

price :

330 USD

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

reactivity :

human, mouse, rat, chicken

application :

western blot, flow cytometry, immunohistochemistry - frozen section

more info or order :

HUABIO product webpage

image

image 1 :

Western blot analysis of iNOS on different lysates with Rabbit anti-iNOS antibody (ER1706-89) at 1/1,000 dilution. Lane 1: RAW264.7 treated with 1μg/mL LPS for 24 hours whole cell lysate (20 µg/Lane) Lane 2: RAW264.7 whole cell lysate (20 µg/Lane) Lane 3: HeLa cell lysate (30 µg/Lane) Lane 4: A549 cell lysate (30 µg/Lane) Predicted band size: 131 kDa Observed band size: 131 kDa Exposure time: 16 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ER1706-89) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:100,000 dilution was used for 1 hour at room temperature.

image 2 :

Immunocytochemistry analysis of RAW264.7 cells labeling iNOS with Rabbit anti-iNOS antibody (ER1706-89) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-iNOS antibody (ER1706-89) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (M1305-2, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution.

image 3 :

Immunocytochemistry analysis of C6 cells labeling iNOS with Rabbit anti-iNOS antibody (ER1706-89) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-iNOS antibody (ER1706-89) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (M1305-2, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution.

product information

SKU :

ER1706-89

Target name :

iNOS

Species reactivity :

Human,Mouse,Rat,Chicken

Applications :

IF-Cell,FC,WB,IHC-Fr

Conjugate :

Non-conjugated

Immunogen :

Synthetic peptide within human iNOS aa 1,104-1,153 / 1,153.

Uniprot id :

P35228>SwissProt: P35228 Human;SwissProt: P29477 Mouse

Host :

Rabbit

Isotype :

IgG

Size :

100μl

List Price :

330 USD

Storage Buffer :

1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.

Form :

Liquid

Storage Instruction :

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.

Purity :

Immunogen affinity purified.

Product type :

Rabbit polyclonal Antibody

Positive control :

RAW264.7 treated with 1μg/mL LPS for 24 hours whole cell lysate, RAW264.7 whole cell lysate, HeLa cell lysate, A549 cell lysate, A549, LOVO.

Molecular wt :

Predicted band size: 131 kDa

Subcellular location :

Cytoskeleton, Nucleus, Cytosol.

Concentration :

1 mg/mL.

Recommended dilutions :

WB: 1:2,000-1:5,000 ;IF-Cell: 1:50-1:200 ;FC: 1:50-1:100 ;IHC-Fr:1:100

Advanced Validation :

Cell treatment (CT)

Pic img4 :

https://storage.huabio.cn/huabio/productImg/ER1706-89_4.jpg

Pic legend4 :

ICC staining iNOS in LOVO cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Pic img5 :

https://storage.huabio.cn/huabio/productImg/ER1706-89_5.jpg

Pic legend5 :

Flow cytometric analysis of LOVO cells with iNOS antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black).

Pic img6 :

https://storage.huabio.cn/huabio/productImg/ER1706-89_6.jpg

Pic legend6 :

Immunofluorescence analysis of frozen mouse hippocampus tissue labeling iNOS with Rabbit anti-iNOS antibody (ER1706-89). The tissues were blocked in 3% BSA for 30 minutes at room temperature, washed with PBS, and then probed with the primary antibody (ER1706-89, green) at 1/100 dilution overnight at 4℃, washed with PBS. Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) was used as the secondary antibody at 1/200 dilution. Nuclei were counterstained with DAPI (blue). Image acquisition was performed with KFBIO KF-FL-400 Scanner.

Pic img7 :

https://storage.huabio.cn/huabio/productImg/ER1706-89_7.jpg

Pic legend7 :

Immunofluorescence analysis of frozen mouse cerebral cortex tissue labeling iNOS with Rabbit anti-iNOS antibody (ER1706-89). The tissues were blocked in 3% BSA for 30 minutes at room temperature, washed with PBS, and then probed with the primary antibody (ER1706-89, green) at 1/100 dilution overnight at 4℃, washed with PBS. Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) was used as the secondary antibody at 1/200 dilution. Nuclei were counterstained with DAPI (blue). Image acquisition was performed with KFBIO KF-FL-400 Scanner.

more info or order :

HUABIO product webpage