ATP citrate lyase | HUABIO ER1706-75 product information

product summary

company name :

HUABIO

product type :

antibody

product name :

ATP citrate lyase

catalog :

ER1706-75

quantity :

100μl

price :

330 USD

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

reactivity :

human, mouse, rat

application :

western blot, flow cytometry, immunohistochemistry - paraffin section

more info or order :

HUABIO product webpage

image

image 1 :

Western blot analysis of ATP citrate lyase on mouse pancreas tissue (1) and NIH-3T3 cell (2) lysate using anti-ATP citrate lyase antibody at 1/200 dilution.

image 2 :

All lanes: Western blot analysis of ATP citrate lyase with anti-ATP citrate lyase antibody (ER1706-75) at 1:500 dilution. Lane 1: Wild-type A549 whole cell lysate (10 µg). Lane 2: ATP citrate lyase knockout A549 whole cell lysate (10 µg). ER1706-75 was shown to specifically react with ATP citrate lyase in wild-type A549 cells. No band was observed when ATP citrate lyase knockout sample was tested. Wild-type and ATP citrate lyase knockout samples were subjected to SDS-PAGE. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM in TBST for 1 hour at room temperature. The primary antibody (ER1706-75, 1:500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG-HRP Secondary Antibody (HA1001) at 1:300,000 dilution was used for 1 hour at room temperature.

image 3 :

ICC staining ATP citrate lyase in A549 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

product information

SKU :

ER1706-75

Target name :

ATP citrate lyase

Species reactivity :

Human,Mouse,Rat

Applications :

WB,IF-Cell,IHC-P,FC

Conjugate :

Non-conjugated

Immunogen :

Recombinant protein within human ATP citrate lyase aa 813-1066.

Uniprot id :

P53396>SwissProt: P53396 Human;SwissProt: Q91V92 Mouse;SwissProt: P16638 Rat

Host :

Rabbit

Isotype :

IgG

Size :

100μl

List Price :

330 USD

Storage Buffer :

1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.

Form :

Liquid

Storage Instruction :

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.

Purity :

Immunogen affinity purified.

Product type :

Rabbit polyclonal Antibody

Positive control :

NIH-3T3, A549, SH-SY5Y, mouse pancreas tissue lysate, rat brain tissue, human colon cancer tissue, human pancreas tissue, mouse testis tissue.

Molecular wt :

Predicted band size: 121 kDa

Subcellular location :

Cytoplasm, cytosol.

Concentration :

1 mg/mL.

Recommended dilutions :

WB: 1:500;IF-Cell: 1:50-1:200;IHC-P: 1:50-1:200;FC: 1:50-1:100

Advanced Validation :

Knockout (KO)

Pic img4 :

https://storage.huabio.cn/huabio/productImg/ER1706-75_4.jpg

Pic legend4 :

ICC staining ATP citrate lyase in SH-SY5Y cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Pic img5 :

https://storage.huabio.cn/huabio/productImg/ER1706-75_5.jpg

Pic legend5 :

Immunohistochemical analysis of paraffin-embedded rat brain tissue using anti-ATP citrate lyase antibody. Counter stained with hematoxylin.

Pic img6 :

https://storage.huabio.cn/huabio/productImg/ER1706-75_6.jpg

Pic legend6 :

Immunohistochemical analysis of paraffin-embedded human colon cancer tissue using anti-ATP citrate lyase antibody. Counter stained with hematoxylin.

Pic img7 :

https://storage.huabio.cn/huabio/productImg/ER1706-75_7.jpg

Pic legend7 :

Immunohistochemical analysis of paraffin-embedded human pancreas tissue using anti-ATP citrate lyase antibody. Counter stained with hematoxylin.

Pic img8 :

https://storage.huabio.cn/huabio/productImg/ER1706-75_8.jpg

Pic legend8 :

Immunohistochemical analysis of paraffin-embedded mouse testis tissue using anti-ATP citrate lyase antibody. Counter stained with hematoxylin.

Pic img9 :

https://storage.huabio.cn/huabio/productImg/ER1706-75_9.jpg

Pic legend9 :

Flow cytometric analysis of A549 cells with ATP citrate lyase antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.

more info or order :

HUABIO product webpage