NLRP3 | HUABIO ER1706-72 product information

product summary

company name :

HUABIO

product type :

antibody

product name :

NLRP3

catalog :

ER1706-72

quantity :

100μl

price :

330 USD

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

reactivity :

human, mouse

application :

western blot, flow cytometry, immunohistochemistry - paraffin section

more info or order :

HUABIO product webpage

image

image 1 :

Western blot analysis of NLRP3 on LOVO cell lysates with Rabbit anti-NLRP3 antibody (ER1706-72) at 1/500 dilution. Lysates/proteins at 30 µg/Lane. Predicted band size: 118 kDa Observed band size: 118 kDa Exposure time: 1 minute; 6% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ER1706-72) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:300,000 dilution was used for 1 hour at room temperature.

image 2 :

ICC staining NLRP3 in A431 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

image 3 :

ICC staining NLRP3 in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

product information

SKU :

ER1706-72

Target name :

NLRP3

Species reactivity :

Human,Mouse

Applications :

WB,IF-Cell,IHC-P,FC

Conjugate :

Non-conjugated

Immunogen :

Synthetic peptide within Human NLRP3 aa 987-1,036 / 1,036.

Uniprot id :

Q96P20>SwissProt: Q96P20 Human;SwissProt: Q8R4B8 Mouse

Host :

Rabbit

Isotype :

IgG

Size :

100μl

List Price :

330 USD

Storage Buffer :

1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.

Form :

Liquid

Storage Instruction :

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.

Purity :

Immunogen affinity purified.

Product type :

Rabbit polyclonal Antibody

Positive control :

LOVO cell lysate, A431, HepG2, LOVO, human liver cancer tissue, human colon cancer tissue, human placenta tissue, mouse brain tissue, mouse kidney tissue, Jurkat.

Molecular wt :

Predicted band size: 118 kDa

Subcellular location :

Nucleus. Cytoplasm. Secreted.

Concentration :

1 mg/mL.

Recommended dilutions :

WB: 1:500-1:2,000 ;IF-Cell: 1:500-1:2,000 ;IHC-P: 1:50-1:200 ;FC: 1:50-1:100

Pic img4 :

https://storage.huabio.cn/huabio/productImg/ER1706-72_4.jpg

Pic legend4 :

ICC staining NLRP3 in LOVO cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Pic img5 :

https://storage.huabio.cn/huabio/productImg/ER1706-72_5.jpg

Pic legend5 :

Immunohistochemical analysis of paraffin-embedded human liver cancer tissue using anti-NLRP3 antibody. Counter stained with hematoxylin.

Pic img6 :

https://storage.huabio.cn/huabio/productImg/ER1706-72_6.jpg

Pic legend6 :

Immunohistochemical analysis of paraffin-embedded human colon cancer tissue using anti-NLRP3 antibody. Counter stained with hematoxylin.

Pic img7 :

https://storage.huabio.cn/huabio/productImg/ER1706-72_7.jpg

Pic legend7 :

Immunohistochemical analysis of paraffin-embedded human placenta tissue using anti-NLRP3 antibody. Counter stained with hematoxylin.

Pic img8 :

https://storage.huabio.cn/huabio/productImg/ER1706-72_8.jpg

Pic legend8 :

Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti-NLRP3 antibody. Counter stained with hematoxylin.

Pic img9 :

https://storage.huabio.cn/huabio/productImg/ER1706-72_9.jpg

Pic legend9 :

Immunohistochemical analysis of paraffin-embedded mouse kidney tissue using anti-NLRP3 antibody. Counter stained with hematoxylin.

Pic img10 :

https://storage.huabio.cn/huabio/productImg/ER1706-72_10.jpg

Pic legend10 :

Flow cytometric analysis of Jurkat cells with NLRP3 antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.

more info or order :

HUABIO product webpage