BMAL1 | HUABIO ER1706-71 product information

product summary

company name :

HUABIO

product type :

antibody

product name :

BMAL1

catalog :

ER1706-71

quantity :

100μl

price :

330 USD

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

reactivity :

human, mouse, rat

application :

western blot, immunohistochemistry - paraffin section

more info or order :

HUABIO product webpage

image

image 1 :

Western blot analysis of BMAL1 on SH-SY5Y cell lysates using anti-BMAL1 antibody at 1/1,000 dilution.

image 2 :

Immunocytochemistry analysis of SH-SY5Y cells labeling BMAL1 with Rabbit anti-BMAL1 antibody (ER1706-71) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-BMAL1 antibody (ER1706-71) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (HA601187, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution.

image 3 :

Immunohistochemical analysis of paraffin-embedded human liver cncer tissue using anti-BMAL1 antibody. Counter stained with hematoxylin.

product information

SKU :

ER1706-71

Target name :

BMAL1

Species reactivity :

Human,Mouse,Rat

Applications :

WB,IF-Cell,IHC-P

Conjugate :

Non-conjugated

Immunogen :

Recombinant protein within N-terminal human BMAL1.

Uniprot id :

O00327>SwissProt: O00327 Human;SwissProt: Q9WTL8 Mouse;SwissProt: Q9EPW1 Rat

Host :

Rabbit

Isotype :

IgG

Size :

100μl

List Price :

330 USD

Storage Buffer :

1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.

Form :

Liquid

Storage Instruction :

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.

Purity :

Immunogen affinity purified.

Product type :

Rabbit polyclonal Antibody

Positive control :

SH-SY5Y cell lysates, SH-SY5Y, human liver cncer tissue, human prostate tissue, mouse brain tissue, mouse prostate tissue, rat brain tissue.

Molecular wt :

Predicted band size: 69 kDa

Subcellular location :

Cytoplasm, Nucleus.

Concentration :

1 mg/mL.

Recommended dilutions :

WB: 1:500-2,000 ;IF-Cell: 1:100-1:500 ;IHC-P: 1:50-1:200

Pic img4 :

https://storage.huabio.cn/huabio/productImg/ER1706-71_4.jpg

Pic legend4 :

Immunohistochemical analysis of paraffin-embedded human prostate tissue using anti-BMAL1 antibody. Counter stained with hematoxylin.

Pic img5 :

https://storage.huabio.cn/huabio/productImg/ER1706-71_5.jpg

Pic legend5 :

Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti-BMAL1 antibody. Counter stained with hematoxylin.

Pic img6 :

https://storage.huabio.cn/huabio/productImg/ER1706-71_6.jpg

Pic legend6 :

Immunohistochemical analysis of paraffin-embedded mouse prostate tissue using anti-BMAL1 antibody. Counter stained with hematoxylin.

Pic img7 :

https://storage.huabio.cn/huabio/productImg/ER1706-71_7.jpg

Pic legend7 :

Immunohistochemical analysis of paraffin-embedded rat brain tissue using anti-BMAL1 antibody. Counter stained with hematoxylin.

more info or order :

HUABIO product webpage