Cullin-3 | HUABIO ER1706-70 product information

product summary

company name :

HUABIO

product type :

antibody

product name :

Cullin-3

catalog :

ER1706-70

quantity :

100μl

price :

330 USD

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

reactivity :

human, mouse

application :

western blot, flow cytometry, immunohistochemistry - paraffin section

more info or order :

HUABIO product webpage

image

image 1 :

Western blot analysis of Cullin 3 on different lysates using anti-Cullin 3 antibody at 1/1,000 dilution. Positive control: Lane 1: SH-SY5Y Lane 2: 293T Lane 3: Mouse testis tissue

image 2 :

Immunohistochemical analysis of paraffin-embedded human kidney tissue with Rabbit anti-Cullin-3 antibody (ER1706-70) at 1/500 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ER1706-70) at 1/500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

image 3 :

Immunohistochemical analysis of paraffin-embedded human placenta tissue using anti-Cullin-3 antibody. Counter stained with hematoxylin.

product information

SKU :

ER1706-70

Target name :

Cullin-3

Species reactivity :

Human,Mouse

Applications :

WB,IHC-P,FC

Conjugate :

Non-conjugated

Immunogen :

Synthetic peptide within human Cullin-3 aa 724-768.

Uniprot id :

Q13618>SwissProt: Q13618 Human;SwissProt: Q9JLV5 Mouse

Host :

Rabbit

Isotype :

IgG

Size :

100μl

List Price :

330 USD

Storage Buffer :

1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.

Form :

Liquid

Storage Instruction :

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.

Purity :

Immunogen affinity purified.

Product type :

Rabbit polyclonal Antibody

Positive control :

SH-SY5Y, 293T, mouse testis tissue lysate, human kidney tissue, human placenta tissue.

Molecular wt :

Predicted band size: 89 kDa

Subcellular location :

Golgi apparatus. Nucleus.

Concentration :

1 mg/mL.

Recommended dilutions :

WB: 1:500-1:2,000 ;IHC-P: 1:50-1:500 ;FC: 1:50-1:100

Pic img4 :

https://storage.huabio.cn/huabio/productImg/ER1706-70_4.jpg

Pic legend4 :

Flow cytometric analysis of SH-SY5Y cells with Cullin 3 antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.

Pic img5 :

https://storage.huabio.cn/huabio/productImg/ER1706-70_5.jpg

Pic legend5 :

Western blot analysis of Cullin-3 on mouse testis tissue lysates with Rabbit anti-Cullin-3 antibody (ER1706-70) at 1/2,000 dilution. Lysates/proteins at 20 µg/Lane. Predicted band size: 89 kDa Observed band size: 80 kDa Exposure time: 2 minutes; 8% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ER1706-70) at 1/2,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:300,000 dilution was used for 1 hour at room temperature.

more info or order :

HUABIO product webpage