AMACR | HUABIO ER1706-60 product information

product summary

company name :

HUABIO

product type :

antibody

product name :

AMACR

catalog :

ER1706-60

quantity :

100μl

price :

330 USD

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

reactivity :

human, mouse

application :

western blot, flow cytometry, immunohistochemistry - paraffin section

more info or order :

HUABIO product webpage

image

image 1 :

Western blot analysis of AMACR on different lysates using anti-AMACR antibody at 1/1,000 dilution. Positive control: Lane 1: Mouse liver tissue Lane 2: SiHa Lane 3: SW480 Lane 4: Human kidney tissue

image 2 :

ICC staining AMACR in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

image 3 :

Immunohistochemical analysis of paraffin-embedded human liver tissue with Rabbit anti-AMACR antibody (ER1706-60) at 1/400 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ER1706-60) at 1/400 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

product information

SKU :

ER1706-60

Target name :

AMACR

Species reactivity :

Human,Mouse

Applications :

WB,IF-Cell,IHC-P,FC

Conjugate :

Non-conjugated

Immunogen :

Recombinant protein within Human AMACR aa 1-382 / 382.

Uniprot id :

Q9UHK6>SwissProt: Q9UHK6 Human;SwissProt: O09174 Mouse

Host :

Rabbit

Isotype :

IgG

Size :

100μl

List Price :

330 USD

Storage Buffer :

1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.

Form :

Liquid

Storage Instruction :

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.

Purity :

Immunogen affinity purified.

Product type :

Rabbit polyclonal Antibody

Positive control :

Human kidney tissue, mouse liver tissue lysate, Siha, SW480, HepG2, human liver tissue, human colon cancer tissue, mouse colon tissue, PC-3M.

Molecular wt :

Predicted band size: 42 kDa

Subcellular location :

Mitochondrion. Peroxisome.

Concentration :

1 mg/mL.

Recommended dilutions :

WB: 1:500 ;IF-Cell: 1:50-1:100 ;IHC-P: 1:50-1:1,000 ;FC: 1:50-1:100

Pic img4 :

https://storage.huabio.cn/huabio/productImg/ER1706-60_4.jpg

Pic legend4 :

Immunohistochemical analysis of paraffin-embedded human colon cancer tissue using anti-AMACR antibody. Counter stained with hematoxylin.

Pic img5 :

https://storage.huabio.cn/huabio/productImg/ER1706-60_5.png

Pic legend5 :

Immunohistochemical analysis of paraffin-embedded human kidney tissue with Rabbit anti-AMACR antibody (ER1706-60) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ER1706-60) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Pic img6 :

https://storage.huabio.cn/huabio/productImg/ER1706-60_6.png

Pic legend6 :

Immunohistochemical analysis of paraffin-embedded mouse colon tissue with Rabbit anti-AMACR antibody (ER1706-60) at 1/400 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ER1706-60) at 1/400 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Pic img7 :

https://storage.huabio.cn/huabio/productImg/ER1706-60_7.jpg

Pic legend7 :

Flow cytometric analysis of PC-3M cells with AMACR antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.

more info or order :

HUABIO product webpage