MSI2 | HUABIO ER1706-59 product information

product summary

company name :

HUABIO

product type :

antibody

product name :

MSI2

catalog :

ER1706-59

quantity :

100μl

price :

330 USD

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

reactivity :

human, mouse, rat

application :

western blot, flow cytometry, immunohistochemistry - paraffin section

more info or order :

HUABIO product webpage

image

image 1 :

Western blot analysis of MSI2 on different lysates with Rabbit anti-MSI2 antibody (ER1706-59) at 1/2,000 dilution. Lane 1: K562 cell lysate Lane 2: PC-12 cell lysate Lysates/proteins at 10 µg/Lane. Predicted band size: 35/37 kDa Observed band size: 35/37 kDa Exposure time: 2 minutes; 12% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ER1706-59) at 1/2,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:300,000 dilution was used for 1 hour at room temperature.

image 2 :

Western blot analysis of MSI2 on different lysates with Rabbit anti-MSI2 antibody (ER1706-59) at 1/1,000 dilution. Lane 1: Hela-si NT cell lysate Lane 2: Hela-si MSI2#1 cell lysate Lane 3: Hela-si MSI2#2 cell lysate Lysates/proteins at 10 µg/Lane. Predicted band size: 35 kDa Observed band size: 35 kDa Exposure time: 50 seconds; 4-20% SDS-PAGE gel. ER1706-59 was shown to specifically react with MSI2 in Hela-si NT cells. No bands were observed when Hela-si MSI2 samples were tested. Hela-si NT and Hela-si MSI2 samples were subjected to SDS-PAGE. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM in TBST for 1 hour at room temperature. The primary antibody (ER1706-59, 1/1,000) and Loading control antibody (Rabbit anti-GAPDH, ET1601-4, 1/10,000) were used in 5% BSA at room temperature for 2 hours. Goat Anti-rabbit IgG-HRP Secondary Antibody (HA1001) at 1:100,000 dilution was used for 1 hour at room temperature.

image 3 :

ICC staining MSI2 in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

product information

SKU :

ER1706-59

Target name :

MSI2

Species reactivity :

Human,Mouse,Rat

Applications :

WB,IF-Cell,IHC-P,FC

Conjugate :

Non-conjugated

Immunogen :

Recombinant protein within Human MSI2 aa 1-185 / 328.

Uniprot id :

Q96DH6>SwissProt: Q96DH6 Human;SwissProt: Q920Q6 Mouse;SwissProt: F1LWE6 Rat

Host :

Rabbit

Isotype :

IgG

Size :

100μl

List Price :

330 USD

Storage Buffer :

1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.

Form :

Liquid

Storage Instruction :

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.

Purity :

Immunogen affinity purified.

Product type :

Rabbit polyclonal Antibody

Positive control :

K562, HepG2, PC-12, SH-SY5Y, rat epididymis tissue, human breast tissue, human placenta tissue, mouse brain tissue.

Molecular wt :

Predicted band size: 35/37 kDa

Subcellular location :

Cytoplasm

Concentration :

1 mg/mL.

Recommended dilutions :

WB: 1:500-1:2,000 ;IF-Cell: 1:50-1:200 ;IHC-P: 1:50-1:4,000 ;FC: 1:50-1:100

Advanced Validation :

Knockdown (KD)

Pic img4 :

https://storage.huabio.cn/huabio/productImg/ER1706-59_4.jpg

Pic legend4 :

ICC staining MSI2 in PC-12 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Pic img5 :

https://storage.huabio.cn/huabio/productImg/ER1706-59_5.jpg

Pic legend5 :

ICC staining MSI2 in SH-SY5Y cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Pic img6 :

https://storage.huabio.cn/huabio/productImg/ER1706-59_6.png

Pic legend6 :

Immunohistochemical analysis of paraffin-embedded rat epididymis tissue with Rabbit anti-MSI2 antibody (ER1706-59) at 1/2,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ER1706-59) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Pic img7 :

https://storage.huabio.cn/huabio/productImg/ER1706-59_7.jpg

Pic legend7 :

Immunohistochemical analysis of paraffin-embedded human breast tissue using anti-MSI2 antibody. Counter stained with hematoxylin.

Pic img8 :

https://storage.huabio.cn/huabio/productImg/ER1706-59_8.png

Pic legend8 :

Immunohistochemical analysis of paraffin-embedded human placenta tissue with Rabbit anti-MSI2 antibody (ER1706-59) at 1/2,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ER1706-59) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Pic img9 :

https://storage.huabio.cn/huabio/productImg/ER1706-59_9.png

Pic legend9 :

Immunohistochemical analysis of paraffin-embedded mouse brain tissue with Rabbit anti-MSI2 antibody (ER1706-59) at 1/4,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ER1706-59) at 1/4,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Pic img10 :

https://storage.huabio.cn/huabio/productImg/ER1706-59_10.jpg

Pic legend10 :

Flow cytometric analysis of SH-SY5Y cells with MSI2 antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.

more info or order :

HUABIO product webpage