JAK2 | HUABIO ER1706-58 product information

product summary

company name :

HUABIO

product type :

antibody

product name :

JAK2

catalog :

ER1706-58

quantity :

100μl

price :

330 USD

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

reactivity :

human, mouse, rat

application :

immunohistochemistry - paraffin section

more info or order :

HUABIO product webpage

image

image 1 :

Immunocytochemistry analysis of HUVEC cells labeling JAK2 with Rabbit anti-JAK2 antibody (ER1706-58) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-JAK2 antibody (ER1706-58) at 1/50 dilution in 2% negative goat serum overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.

image 2 :

ICC staining JAK2 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

image 3 :

Immunohistochemical analysis of paraffin-embedded human kidney tissue with Rabbit anti-JAK2 antibody (ER1706-58) at 1/400 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ER1706-58) at 1/400 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

product information

SKU :

ER1706-58

Target name :

JAK2

Species reactivity :

Human,Mouse,Rat

Applications :

IF-Cell,IHC-P

Conjugate :

Non-conjugated

Immunogen :

Synthetic peptide within C-terminal human JAK2.

Uniprot id :

O60674>SwissProt: O60674 Human;SwissProt: Q62120 Mouse;SwissProt: Q62689 Rat

Host :

Rabbit

Isotype :

IgG

Size :

100μl

List Price :

330 USD

Storage Buffer :

1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.

Form :

Liquid

Storage Instruction :

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.

Purity :

Immunogen affinity purified.

Product type :

Rabbit polyclonal Antibody

Positive control :

Hela, HUVEC, rat liver tissue, human tonsil tissue, human colon cancer tissue, human kidney tissue, mouse kidney tissue.

Molecular wt :

Predicted band size: 131 kDa

Subcellular location :

Cytoplasm, Nucleus.

Concentration :

1 mg/mL.

Recommended dilutions :

IF-Cell: 1:50-1:200 ;IHC-P: 1:50-1:400

Pic img4 :

https://storage.huabio.cn/huabio/productImg/ER1706-58_4.png

Pic legend4 :

Immunohistochemical analysis of paraffin-embedded mouse kidney tissue with Rabbit anti-JAK2 antibody (ER1706-58) at 1/400 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ER1706-58) at 1/400 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Pic img5 :

https://storage.huabio.cn/huabio/productImg/ER1706-58_5.jpg

Pic legend5 :

Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-JAK2 antibody. Counter stained with hematoxylin.

Pic img6 :

https://storage.huabio.cn/huabio/productImg/ER1706-58_6.jpg

Pic legend6 :

Immunohistochemical analysis of paraffin-embedded human colon cancer tissue using anti-JAK2 antibody. Counter stained with hematoxylin.

Pic img7 :

https://storage.huabio.cn/huabio/productImg/ER1706-58_7.jpg

Pic legend7 :

Immunohistochemical analysis of paraffin-embedded rat liver tissue using anti-JAK2 antibody. Counter stained with hematoxylin.

more info or order :

HUABIO product webpage