IL-4 | HUABIO ER1706-55 product information

product summary

company name :

HUABIO

product type :

antibody

product name :

IL-4

catalog :

ER1706-55

quantity :

100μl

price :

330 USD

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

reactivity :

human

application :

western blot, immunohistochemistry - paraffin section

more info or order :

HUABIO product webpage

image

image 1 :

Western blot analysis of IL4 on recombiant protein lysate using anti-IL4 antibody at 1/1,000 dilution.

image 2 :

Immunocytochemistry analysis of Ramos cells labeling IL-4 with Rabbit anti-IL-4 antibody (ER1706-55) at 1/250 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-IL-4 antibody (ER1706-55) at 1/250 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (HA601187, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution.

image 3 :

Immunohistochemical analysis of paraffin-embedded human breast cancer tissue using anti-IL4 antibody. Counter stained with hematoxylin.

product information

SKU :

ER1706-55

Target name :

IL-4

Species reactivity :

Human

Applications :

WB,IF-Cell,IHC-P

Conjugate :

Non-conjugated

Immunogen :

Recombinant protein within human IL4 aa 25-153.

Uniprot id :

P05112>SwissProt: P05112 Human

Host :

Rabbit

Isotype :

IgG

Size :

100μl

List Price :

330 USD

Storage Buffer :

1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.

Form :

Liquid

Storage Instruction :

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.

Purity :

Immunogen affinity purified.

Product type :

Rabbit polyclonal Antibody

Positive control :

Ramos, human breast cancer tissue, human spleen tissue.

Molecular wt :

Predicted band size: 17 kDa

Subcellular location :

Secreted.

Concentration :

1 mg/mL.

Recommended dilutions :

WB: 1:500-1:1,000 ;IF-Cell: 1:250 ;IHC-P: 1:50-1:600

Pic img4 :

https://storage.huabio.cn/huabio/productImg/ER1706-55_4.png

Pic legend4 :

Immunohistochemical analysis of paraffin-embedded human spleen tissue using anti-IL4 antibody. Counter stained with hematoxylin.

more info or order :

HUABIO product webpage