HMGB2 | HUABIO ER1706-52 product information

product summary

company name :

HUABIO

product type :

antibody

product name :

HMGB2

catalog :

ER1706-52

quantity :

100μl

price :

330 USD

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

reactivity :

human, mouse, rat

application :

western blot, immunohistochemistry - paraffin section

more info or order :

HUABIO product webpage

image

image 1 :

Western blot analysis of HMGB2 on different lysates with Rabbit anti-HMGB2 antibody (ER1706-52) at 1/1,000 dilution. Lane 1: HAP1-parental cell lysate Lane 2: HAP1-HMGB2 KD cell lysate Lysates/proteins at 10 µg/Lane. Predicted band size: 24 kDa Observed band size: 24 kDa Exposure time: 9 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ER1706-52) at 1/1,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

image 2 :

Western blot analysis of HMGB2 on mouse testis tissue lysate using anti-HMGB2 antibody at 1/500 dilution.

image 3 :

ICC staining HMGB2 in LOVO cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

product information

SKU :

ER1706-52

Target name :

HMGB2

Species reactivity :

Human,Mouse,Rat

Applications :

WB,IF-Cell,IHC-P

Conjugate :

Non-conjugated

Immunogen :

Recombinant protein within human HMGB2 aa 22-172.

Uniprot id :

P26583>SwissProt: P26583 Human;SwissProt: P30681 Mouse;SwissProt: P52925 Rat

Host :

Rabbit

Isotype :

IgG

Size :

100μl

List Price :

330 USD

Storage Buffer :

1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.

Form :

Liquid

Storage Instruction :

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.

Purity :

Immunogen affinity purified.

Product type :

Rabbit polyclonal Antibody

Positive control :

Mouse testis tissue, LOVO, SH-SY5Y, rat brain tissue, human tonsil tissue, human kidney tissue.

Molecular wt :

Predicted band size: 24 kDa

Subcellular location :

Secreted. Nucleus. Cytoplasm.

Concentration :

1 mg/mL.

Recommended dilutions :

WB: 1:500-1:1,000 ;IF-Cell: 1:50-1:200 ;IHC-P: 1:50-1:200

Advanced Validation :

Knockdown (KD)

Pic img4 :

https://storage.huabio.cn/huabio/productImg/ER1706-52_4.jpg

Pic legend4 :

ICC staining HMGB2 in SH-SY5Y cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Pic img5 :

https://storage.huabio.cn/huabio/productImg/ER1706-52_5.jpg

Pic legend5 :

Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-HMGB2 antibody. Counter stained with hematoxylin.

Pic img6 :

https://storage.huabio.cn/huabio/productImg/ER1706-52_6.jpg

Pic legend6 :

Immunohistochemical analysis of paraffin-embedded rat brain tissue using anti-HMGB2 antibody. Counter stained with hematoxylin.

Pic img7 :

https://storage.huabio.cn/huabio/productImg/ER1706-52_7.jpg

Pic legend7 :

Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-HMGB2 antibody. Counter stained with hematoxylin.

Pic img8 :

https://storage.huabio.cn/huabio/productImg/ER1706-52_8.jpg

Pic legend8 :

Immunohistochemical analysis of paraffin-embedded mouse testis tissue using anti-HMGB2 antibody. Counter stained with hematoxylin.

more info or order :

HUABIO product webpage