Superoxide Dismutase 1 | HUABIO ER1706-49 product information

product summary

company name :

HUABIO

product type :

antibody

product name :

Superoxide Dismutase 1

catalog :

ER1706-49

quantity :

100μl

price :

330 USD

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

reactivity :

human, mouse, rat

application :

western blot, flow cytometry

more info or order :

HUABIO product webpage

image

image 1 :

Western blot analysis of Superoxide Dismutase 1 on different lysates with Rabbit anti-Superoxide Dismutase 1 antibody (ER1706-49) at 1/1,000 dilution. Lane 1: MCF7 cell lysate (20 µg/Lane) Lane 2: HepG2 cell lysate (20 µg/Lane) Lane 3: HUVEC cell lysate (20 µg/Lane) Lane 4: SH-SY5Y cell lysate (20 µg/Lane) Lane 5: Human liver tissue lysate (40 µg/Lane) Predicted band size: 16 kDa Observed band size: 16 kDa Exposure time: 1 second; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ER1706-49) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

image 2 :

ICC staining SOD1 in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

image 3 :

ICC staining SOD1 in LOVO cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

product information

SKU :

ER1706-49

Target name :

Superoxide Dismutase 1

Species reactivity :

Human,Mouse,Rat

Applications :

WB,IF-Cell,FC

Conjugate :

Non-conjugated

Immunogen :

Recombinant protein within human SOD1 aa 6-154.

Uniprot id :

P00441>SwissProt: P00441 Human;SwissProt: P08228 Mouse;SwissProt: P07632 Rat

Host :

Rabbit

Isotype :

IgG

Size :

100μl

List Price :

330 USD

Storage Buffer :

1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.

Form :

Liquid

Storage Instruction :

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.

Purity :

Immunogen affinity purified.

Product type :

Rabbit polyclonal Antibody

Positive control :

MCF7 cell lysate, HepG2 cell lysate, HUVEC cell lysate, SH-SY5Y cell lysate, human liver tissue lysate, HepG2, LOVO, MCF-7.

Molecular wt :

Predicted band size: 16 kDa

Subcellular location :

Cytoplasm, Mitochondrion, Nucleus.

Concentration :

1 mg/mL.

Recommended dilutions :

WB: 1:1,000 ;IF-Cell: 1:500-1:2,000 ;FC: 1:50-1:100

Pic img4 :

https://storage.huabio.cn/huabio/productImg/ER1706-49_4.jpg

Pic legend4 :

Flow cytometric analysis of MCF-7 cells with SOD1 antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.

more info or order :

HUABIO product webpage