NaV1.7 | HUABIO ER1706-44 product information

product summary

company name :

HUABIO

product type :

antibody

product name :

NaV1.7

catalog :

ER1706-44

quantity :

100μl

price :

330 USD

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

reactivity :

human

application :

flow cytometry, immunohistochemistry - paraffin section

more info or order :

HUABIO product webpage

image

image 1 :

Immunocytochemistry analysis of A549 cells labeling NaV1.7 with Rabbit anti-NaV1.7 antibody (ER1706-44) at 1/500 dilution. Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-NaV1.7 antibody (ER1706-44) at 1/500 dilution in 2% negative goat serum overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.

image 2 :

Immunocytochemistry analysis of HepG2 cells labeling NaV1.7 with Rabbit anti-NaV1.7 antibody (ER1706-44) at 1/500 dilution. Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-NaV1.7 antibody (ER1706-44) at 1/500 dilution in 2% negative goat serum overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.

image 3 :

ICC staining NaV1.7 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

product information

SKU :

ER1706-44

Target name :

NaV1.7

Species reactivity :

Human

Applications :

IF-Cell,IHC-P,FC

Conjugate :

Non-conjugated

Immunogen :

Synthetic peptide within Human NaV17 aa 1-50 / 1,988.

Uniprot id :

Q15858>SwissProt: Q15858 Human

Host :

Rabbit

Isotype :

IgG

Size :

100μl

List Price :

330 USD

Storage Buffer :

1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.

Form :

Liquid

Storage Instruction :

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.

Purity :

Immunogen affinity purified.

Product type :

Rabbit polyclonal Antibody

Positive control :

A549, Hela, HepG2, SH-SY5Y, human liver cancer tissue, mouse colon tissue.

Molecular wt :

Predicted band size: 226 kDa

Subcellular location :

Cell membrane. Cell projection.

Concentration :

1 mg/mL.

Recommended dilutions :

IHC-P: 1:50-1:1,000;IF-Cell: 1:50-1:500;FC: 1:50-1:100

Pic img4 :

https://storage.huabio.cn/huabio/productImg/ER1706-44_4.jpg

Pic legend4 :

ICC staining NaV1.7 in SH-SY5Y cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Pic img5 :

https://storage.huabio.cn/huabio/productImg/ER1706-44_5.png

Pic legend5 :

Immunohistochemical analysis of paraffin-embedded human liver cancer tissue using anti-NaV1.7 beta antibody. Counter stained with hematoxylin.

Pic img6 :

https://storage.huabio.cn/huabio/productImg/ER1706-44_6.png

Pic legend6 :

Immunohistochemical analysis of paraffin-embedded mouse colon tissue using anti-NaV1.7 beta antibody. Counter stained with hematoxylin.

Pic img7 :

https://storage.huabio.cn/huabio/productImg/ER1706-44_7.jpg

Pic legend7 :

Flow cytometric analysis of SH-SY5Y cells with NaV1.7 antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black).

more info or order :

HUABIO product webpage