Nrf2 | HUABIO ER1706-41 product information

product summary

company name :

HUABIO

product type :

antibody

product name :

Nrf2

catalog :

ER1706-41

quantity :

100μl

price :

330 USD

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

reactivity :

human, mouse, rat

application :

western blot, immunohistochemistry - paraffin section

more info or order :

HUABIO product webpage

image

image 1 :

Immunocytochemistry analysis of HCT 116 cells treated with or without 10 μM MG-132 for 6 hours labeling Nrf2 with Rabbit anti-Nrf2 antibody (ER1706-41) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-Nrf2 antibody (ER1706-41) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (M1305-2, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution.

image 2 :

Immunocytochemistry analysis of MEF cells treated with or without 10 μM MG-132 for 6 hours labeling Nrf2 with Rabbit anti-Nrf2 antibody (ER1706-41) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-Nrf2 antibody (ER1706-41) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (M1305-2, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution.

image 3 :

Immunohistochemical analysis of paraffin-embedded rat brain tissue using anti-Nrf2 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ER1706-41, 1/200) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

product information

SKU :

ER1706-41

Target name :

Nrf2

Species reactivity :

Human,Mouse,Rat

Applications :

IHC-P,IF-Cell,WB

Conjugate :

Non-conjugated

Immunogen :

Synthetic peptide within N-terminal human Nrf2.

Uniprot id :

Q16236>SwissProt: Q16236 Human;SwissProt: Q60795 Mouse;SwissProt: O54968 Rat

Host :

Rabbit

Isotype :

IgG

Size :

100μl

List Price :

330 USD

Storage Buffer :

1*TBS (pH7.4), 0.5% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.

Form :

Liquid

Storage Instruction :

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.

Purity :

Immunogen affinity purified.

Product type :

Rabbit polyclonal Antibody

Positive control :

Hela cell lysates, HCT116 cell lysates, HCT 116 cells treated with 10 μM MG-132 for 6 hours, MEF cells treated with 10 μM MG-132 for 6 hours, rat brain tissue, human lung tissue, mouse fallopian tubes tissue, human spleen tissue, human uterus tissue.

Molecular wt :

Predicted band size: 68 kDa

Subcellular location :

Cytoplasm. Nucleus.

Concentration :

1 mg/mL.

Recommended dilutions :

IF-Cell: 1:100 ;IHC-P: 1:50-1:200 ;WB: 1:5,000

Advanced Validation :

Cell treatment (CT)

Pic img4 :

https://storage.huabio.cn/huabio/productImg/ER1706-41_4.jpg

Pic legend4 :

Immunohistochemical analysis of paraffin-embedded human lung tissue using anti-Nrf2 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ER1706-41, 1/100) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Pic img5 :

https://storage.huabio.cn/huabio/productImg/ER1706-41_5.jpg

Pic legend5 :

Immunohistochemical analysis of paraffin-embedded mouse fallopian tubes tissue using anti-Nrf2 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ER1706-41, 1/200) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Pic img6 :

https://storage.huabio.cn/huabio/productImg/ER1706-41_6.jpg

Pic legend6 :

Immunohistochemical analysis of paraffin-embedded human spleen tissue using anti-Nrf2 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ER1706-41, 1/200) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Pic img7 :

https://storage.huabio.cn/huabio/productImg/ER1706-41_7.jpg

Pic legend7 :

Immunohistochemical analysis of paraffin-embedded human uterus tissue using anti-Nrf2 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ER1706-41, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Pic img8 :

https://storage.huabio.cn/huabio/productImg/ER1706-41_8.jpg

Pic legend8 :

Western blot analysis of Nrf2 on different lysates with Rabbit anti-Nrf2 antibody (ER1706-41) at 1/5,000 dilution. Lane 1: HCT 116 cell lysate Lane 2: HCT 116 treated with 25μM MG-132 for 4 hours cell lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 68 kDa Observed band size: 100 kDa Exposure time: 1 minute 33 seconds; ECL: K1802; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ER1706-41) at 1/5,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

more info or order :

HUABIO product webpage