product summary
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company name :
HUABIO
product type :
antibody
product name :
GRP94
catalog :
ER1511-5
quantity :
100μl
price :
330 USD
clonality :
polyclonal
host :
domestic rabbit
conjugate :
nonconjugated
reactivity :
human, mouse, rat
application :
western blot, immunohistochemistry - paraffin section
more info or order :
image
image 1 :
Western blot analysis of GRP94 on different lysates with Rabbit anti-GRP94 antibody (ER1511-5) at 1/1,000 dilution.
Lane 1: HepG2 cell lysate (10 µg/Lane)
Lane 2: HeLa cell lysate (10 µg/Lane)
Lane 3: Human brain tissue lysate (20 µg/Lane)
Lane 4: NIH/3T3 cell lysate (10 µg/Lane)
Lane 5: Mouse placenta tissue lysate (20 µg/Lane)
Lane 6: Mouse brain tissue lysate (20 µg/Lane)
Lane 7: PC-12 cell lysate (10 µg/Lane)
Lane 8: Rat placenta tissue lysate (20 µg/Lane)
Lane 9: Rat brain tissue lysate (20 µg/Lane)
Predicted band size: 92 kDa
Observed band size: 94 kDa
Exposure time: 25 seconds;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ER1511-5) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
image 2 :
Immunocytochemistry analysis of HeLa cells labeling GRP94 with Rabbit anti-GRP94 antibody (ER1511-5) at 1/500 dilution.
Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-GRP94 antibody (ER1511-5) at 1/500 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.
Beta tubulin (HA601187, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution.
image 3 :
Immunocytochemistry analysis of NIH/3T3 cells labeling GRP94 with Rabbit anti-GRP94 antibody (ER1511-5) at 1/500 dilution.
Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-GRP94 antibody (ER1511-5) at 1/500 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.
Beta tubulin (HA601187, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution.
product information
SKU :
ER1511-5
Target name :
GRP94
Species reactivity :
Human,Mouse,Rat
Applications :
WB,IF-Cell,IHC-P
Conjugate :
Non-conjugated
Immunogen :
Synthetic peptide within human GRP94 aa 194-247.
Uniprot id :
P14625>SwissProt: P14625 Human;SwissProt: P08113 Mouse;SwissProt: Q66HD0 Rat
Host :
Rabbit
Isotype :
IgG
Size :
100μl
List Price :
330 USD
Storage Buffer :
1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
Form :
Liquid
Storage Instruction :
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
Purity :
Immunogen affinity purified.
Product type :
Rabbit polyclonal Antibody
Positive control :
HepG2 cell lysate, HeLa cell lysate, human brain tissue lysate, NIH/3T3 cell lysate, mouse placenta tissue lysate, mouse brain tissue lysate, PC-12 cell lysate, rat placenta tissue lysate, rat brain tissue lysate, HeLa, NIH/3T3, PC-12 , human liver tissue, human breast tissue, human stomach cancer tissue, mouse stomach tissue.
Molecular wt :
Predicted band size: 92 kDa
Subcellular location :
Endoplasmic reticulum lumen, Melanosome.
Concentration :
1 mg/mL.
Recommended dilutions :
WB: 1:500-1:2,000
;IF-Cell: 1:500
;IHC-P: 1:100-1:1,000
Pic img4 :
https://storage.huabio.cn/huabio/productImg/ER1511-5_4.jpg
Pic legend4 :
Immunocytochemistry analysis of PC-12 cells labeling GRP94 with Rabbit anti-GRP94 antibody (ER1511-5) at 1/500 dilution.
Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-GRP94 antibody (ER1511-5) at 1/500 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.
Beta tubulin (HA601187, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution.
Pic img5 :
https://storage.huabio.cn/huabio/productImg/ER1511-5_5.jpg
Pic legend5 :
Immunohistochemical analysis of paraffin-embedded human liver tissue with Rabbit anti-GRP94 antibody (ER1511-5) at 1/1,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ER1511-5) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Pic img6 :
https://storage.huabio.cn/huabio/productImg/ER1511-5_6.jpg
Pic legend6 :
Immunohistochemical analysis of paraffin-embedded human breast tissue using anti-GRP94 antibody. Counter stained with hematoxylin.
Pic img7 :
https://storage.huabio.cn/huabio/productImg/ER1511-5_7.jpg
Pic legend7 :
Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue using anti-GRP94 antibody. Counter stained with hematoxylin.
Pic img8 :
https://storage.huabio.cn/huabio/productImg/ER1511-5_8.jpg
Pic legend8 :
Immunohistochemical analysis of paraffin-embedded mouse stomach tissue using anti-GRP94 antibody. Counter stained with hematoxylin.
more info or order :
company information
HUABIO
Founded in 2007, HUABIO is dedicated to developing high-quality antibodies that advance innovation. We are passionate about the accuracy, efficiency, and consistency of our products. That is why we have invested in new production platforms, like recombinant rabbit monoclonals, alpaca nanobodies, and adopted aggressive QA standards to deliver cutting-edge antibodies with uncompromised quality.
We hope to see you at your next discovery!
We hope to see you at your next discovery!
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