NOX2 / gp91phox | HUABIO ER1511-34 product information

product summary

company name :

HUABIO

product type :

antibody

product name :

NOX2 / gp91phox

catalog :

ER1511-34

quantity :

100μl

price :

330 USD

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

reactivity :

human, mouse

application :

western blot, flow cytometry, immunohistochemistry - paraffin section

more info or order :

HUABIO product webpage

image

image 1 :

Western blot analysis of NOX2 / gp91phox on different lysates with Rabbit anti-NOX2 / gp91phox antibody (ER1511-34) at 1/2,000 dilution. Lane 1: RAW264.7 cell lysate (20 µg/Lane) Lane 2: Mouse spleen tissue lysate (40 µg/Lane) Lane 3: Mouse skeletal muscle tissue lysate (negative) (40 µg/Lane) Lane 4: Rat spleen tissue lysate (40 µg/Lane) Predicted band size: 65 kDa Observed band size: 55 kDa Exposure time: 20 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ER1511-34) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

image 2 :

Immunocytochemistry analysis of RAW264.7 cells labeling NOX2 / gp91phox with Rabbit anti-NOX2 / gp91phox antibody (ER1511-34) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-NOX2 / gp91phox antibody (ER1511-34) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (HA601187, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution.

image 3 :

Immunohistochemical analysis of paraffin-embedded human liver tissue using anti-NOX2/gp91phox antibody. Counter stained with hematoxylin.

product information

SKU :

ER1511-34

Target name :

NOX2 / gp91phox

Species reactivity :

Human,Mouse

Applications :

IF-Cell,IHC-P,FC,WB

Conjugate :

Non-conjugated

Immunogen :

Synthetic peptide corresponding to C-terminal human NOX2.

Uniprot id :

P04839>SwissProt: P04839 Human;SwissProt: Q61093 Mouse

Host :

Rabbit

Isotype :

IgG

Size :

100μl

List Price :

330 USD

Storage Buffer :

1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.

Form :

Liquid

Storage Instruction :

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.

Purity :

Immunogen affinity purified.

Product type :

Rabbit polyclonal Antibody

Positive control :

RAW264.7 cell lysate, mouse spleen tissue lysate, rat spleen tissue lysate, RAW264.7, human liver tissue, human spleen tissue, human tonsil tissue, mouse spleen tissue.

Molecular wt :

Predicted band size: 65 kDa

Subcellular location :

Cell membrane

Concentration :

1 mg/mL.

Recommended dilutions :

IF-Cell: 1:50-1:200 ;IHC-P: 1:500-1:1,000 ;FC: 1:50-1:100 ;WB: 1:2,000

Advanced Validation :

Relative expression (RE)

Pic img4 :

https://storage.huabio.cn/huabio/productImg/ER1511-34_4.jpg

Pic legend4 :

Immunohistochemical analysis of paraffin-embedded human spleen tissue using anti-NOX2/gp91phox antibody. Counter stained with hematoxylin.

Pic img5 :

https://storage.huabio.cn/huabio/productImg/ER1511-34_5.jpg

Pic legend5 :

Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-NOX2/gp91phox antibody. Counter stained with hematoxylin.

Pic img6 :

https://storage.huabio.cn/huabio/productImg/ER1511-34_6.jpg

Pic legend6 :

Immunohistochemical analysis of paraffin-embedded mouse spleen tissue using anti-NOX2/gp91phox antibody. Counter stained with hematoxylin.

Pic img7 :

https://storage.huabio.cn/huabio/productImg/ER1511-34_7.jpg

Pic legend7 :

Flow cytometric analysis of Hela cells with NOX2/gp91phox antibody at 1/100 dilution (blue) compared with an unlabelled control (cells without incubation with primary antibody; red). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.

more info or order :

HUABIO product webpage