BDNF | HUABIO ER130915 product information

product summary

company name :

HUABIO

product type :

antibody

product name :

BDNF

catalog :

ER130915

quantity :

100μl

price :

330 USD

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

reactivity :

human, mouse, rat

application :

western blot, immunohistochemistry - paraffin section

more info or order :

HUABIO product webpage

image

image 1 :

Western blot analysis of BDNF on different cell lysates using anti-BDNF antibody at 1/500 dilution. Positive control: Lane 1: A172 cell lysates Lane 2: SHG-44 cell lysates Lane 3: Mouse heart tissue lysates Lane 4: Mouse brain tissue lysates

image 2 :

Western blot analysis of BDNF on rat spinal cord tissue lysates with Rabbit anti-BDNF antibody (ER130915) at 1/500 dilution. Lysates/proteins at 20 µg/Lane. Predicted band size: 28 kDa Observed band size: 28 kDa Exposure time: 2 minutes; 12% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ER130915) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:300,000 dilution was used for 1 hour at room temperature.

image 3 :

ICC staining of BNDF in SHG-44 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

product information

SKU :

ER130915

Target name :

BDNF

Species reactivity :

Human,Mouse,Rat

Applications :

WB,IF-Cell,IHC-P

Conjugate :

Non-conjugated

Immunogen :

Synthetic peptide within N-terminal human BDNF.

Uniprot id :

P23560>SwissProt: P23560 Human;SwissProt: P21237 Mouse;SwissProt: P23363 Rat

Host :

Rabbit

Isotype :

IgG

Size :

100μl

List Price :

330 USD

Storage Buffer :

1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Form :

Liquid

Storage Instruction :

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.

Purity :

Immunogen affinity purified.

Product type :

Rabbit polyclonal Antibody

Positive control :

A172 cell lysates, SHG-44 cell lysates, mouse heart tissue lysates, mouse brain tissue lysates, SHG-44, human lung tissue, mouse lung tissue, mouse testis tissue, mouse heart tissue, rat spinal cord tissue lysates

Molecular wt :

Predicted band size: 28 kDa

Subcellular location :

Secreted.

Concentration :

1 mg/mL.

Recommended dilutions :

WB: 1:500 ;IF-Cell: 1:200 ;IHC-P: 1:200

Pic img4 :

https://storage.huabio.cn/huabio/productImg/ER130915_4.jpg

Pic legend4 :

Immunohistochemical analysis of paraffin-embedded human lung tissue with Rabbit anti-BDNF antibody (ER130915) at 1/50 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ER130915) at 1/50 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Pic img5 :

https://storage.huabio.cn/huabio/productImg/ER130915_5.jpg

Pic legend5 :

Immunohistochemical analysis of paraffin-embedded mouse lung tissue with Rabbit anti-BDNF antibody (ER130915) at 1/50 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ER130915) at 1/50 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Pic img6 :

https://storage.huabio.cn/huabio/productImg/ER130915_6.jpg

Pic legend6 :

Immunohistochemical analysis of paraffin-embedded mouse testis tissue with Rabbit anti-BDNF antibody (ER130915) at 1/50 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ER130915) at 1/50 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Pic img7 :

https://storage.huabio.cn/huabio/productImg/ER130915_7.jpg

Pic legend7 :

Immunohistochemical analysis of paraffin-embedded mouse heart tissue with Rabbit anti-BDNF antibody (ER130915) at 1/50 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ER130915) at 1/50 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

more info or order :

HUABIO product webpage