SIRT1 | HUABIO ER130811 product information

product summary

company name :

HUABIO

product type :

antibody

product name :

SIRT1

catalog :

ER130811

quantity :

100μl

price :

330 USD

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

reactivity :

human, mouse

application :

western blot, flow cytometry, immunohistochemistry - paraffin section

more info or order :

HUABIO product webpage

image

image 1 :

Western blot analysis of SIRT1 on different lysates with Rabbit anti-SIRT1 antibody (ER130811) at 1/500 dilution. Lane 1: Hela cell lysate Lane 2: Jurkat cell lysate Lysates/proteins at 10 µg/Lane. Predicted band size: 82 kDa Observed band size: 110 kDa Exposure time: 2 minutes; 6% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ER130811) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:300,000 dilution was used for 1 hour at room temperature.

image 2 :

Western blot analysis of SIRT1 on F9 cell lysates with Rabbit anti-SIRT1 antibody (ER130811) at 1/2,000 dilution. Lysates/proteins at 10 µg/Lane. Predicted band size: 82 kDa Observed band size: 110 kDa Exposure time: 1 minute; 8% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ER130811) at 1/2,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:300,000 dilution was used for 1 hour at room temperature.

image 3 :

Immunocytochemistry analysis of HeLa cells labeling SIRT1 with Rabbit anti-SIRT1 antibody (ER130811) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-SIRT1 antibody (ER130811) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (M1305-2, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution.

product information

SKU :

ER130811

Target name :

SIRT1

Species reactivity :

Human,Mouse

Applications :

WB,IHC-P,FC,IF-Cell

Conjugate :

Non-conjugated

Immunogen :

Synthetic peptide within Human SIRT1 aa 698-747 / 747.

Uniprot id :

Q96EB6>SwissProt: Q96EB6 Human

Host :

Rabbit

Isotype :

IgG

Size :

100μl

List Price :

330 USD

Storage Buffer :

1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Form :

Liquid

Storage Instruction :

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.

Purity :

Immunogen affinity purified.

Product type :

Rabbit polyclonal Antibody

Positive control :

Hela cell lysate, Jurkat cell lysate, F9 cell lysate, HeLa, F9, human colon carcinoma tissue, human lung carcinoma tissue, mouse liver tissue, mouse testis tissue.

Molecular wt :

Predicted band size: 82 kDa

Subcellular location :

Nucleus, cytoplasm, Mitochondrion.

Concentration :

1 mg/mL.

Recommended dilutions :

WB: 1:1,000-1:2,000 ;IHC-P: 1:200 ;FC: 1:1,000 ;IF-Cell: 1:100

Advanced Validation :

Knockdown (KD)

Pic img4 :

https://storage.huabio.cn/huabio/productImg/ER130811_4.jpg

Pic legend4 :

Immunocytochemistry analysis of F9 cells labeling SIRT1 with Rabbit anti-SIRT1 antibody (ER130811) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-SIRT1 antibody (ER130811) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (M1305-2, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution.

Pic img5 :

https://storage.huabio.cn/huabio/productImg/ER130811_5.jpg

Pic legend5 :

Immunohistochemical analysis of paraffin-embedded human colon carcinoma tissue using anti-SIRT1 antibody. Counter stained with hematoxylin.

Pic img6 :

https://storage.huabio.cn/huabio/productImg/ER130811_6.jpg

Pic legend6 :

Immunohistochemical analysis of paraffin-embedded human lung carcinoma tissue using anti-SIRT1 antibody. Counter stained with hematoxylin.

Pic img7 :

https://storage.huabio.cn/huabio/productImg/ER130811_7.jpg

Pic legend7 :

Immunohistochemical analysis of paraffin-embedded mouse liver tissue with Rabbit anti-SIRT1 antibody (ER130811) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ER130811) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Pic img8 :

https://storage.huabio.cn/huabio/productImg/ER130811_8.jpg

Pic legend8 :

Immunohistochemical analysis of paraffin-embedded mouse testis tissue with Rabbit anti-SIRT1 antibody (ER130811) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ER130811) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Pic img9 :

https://storage.huabio.cn/huabio/productImg/ER130811_9.jpg

Pic legend9 :

Flow cytometric analysis of F9 cells labeling SIRT1. Cells were fixed and permeabilized. Then stained with the primary antibody (ER130811, 1/1,000) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).

Pic img10 :

https://storage.huabio.cn/huabio/productImg/ER130811_10.jpg

Pic legend10 :

Western blot analysis of SIRT1 on different lysates with Rabbit anti-SIRT1 antibody (ER130811) at 1/20,000 dilution. Lane 1: HEK-293-si NT cell lysate Lane 2: HEK-293-si SIRT1 cell lysate Lysates/proteins at 10 µg/Lane. Predicted band size: 82 kDa Observed band size: 110 kDa Exposure time: 50 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ER130811) at 1/20,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/100,000 dilution was used for 1 hour at room temperature.

more info or order :

HUABIO product webpage