HMGB1 | HUABIO ER0913 product information

product summary

company name :

HUABIO

product type :

antibody

product name :

HMGB1

catalog :

ER0913

quantity :

100μl

price :

330 USD

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

reactivity :

human

application :

western blot, flow cytometry, immunohistochemistry - paraffin section

more info or order :

HUABIO product webpage

image

image 1 :

All lanes: Western blot analysis of HMGB1 with anti-HMGB1 antibody (ER0913) at 1/500 dilution. Lane 1: Wild-type Raw264.7 whole cell lysate. Lane 2: HMGB1 knockdown Raw264.7 whole cell lysate. ER0913 was shown to specifically react with HMGB1 in Wild-type Raw264.7 cells. Weakened band was observed when HMGB1 knockdown sample was tested. Wild-type and HMGB1 knockdown samples were subjected to SDS-PAGE. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM in TBST for 1 hour at room temperature. The primary Anti-HMGB1 antibody (ER0913, 1/500) and Anti-HSP90 antibody (ET1605-56, 1/10,000) were used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG H&L (HRP) Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature. Cell lysate was provided by Ubigene Biosciences (Ubigene Biosciences Co., Ltd., Guangzhou, China).

image 2 :

Western blot analysis of HMGB1 on different cell lysates using anti- HMGB1 antibody at 1/500 dilution. Positive control: Lane 1: HepG2 cell lysate Lane 2: MCF-7 cell lysate Lane 3: F9 cell lysate Lane 4: A549 cell lysate

image 3 :

ICC staining HMGB1 in Hela cells (green). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

product information

SKU :

ER0913

Target name :

HMGB1

Species reactivity :

Human,Mouse,Rat

Applications :

WB,IF-Cell,IHC-P,FC

Conjugate :

Non-conjugated

Immunogen :

Synthetic peptide within human HMGB1 aa 71-126.

Uniprot id :

P09429>SwissProt: P09429 Human

Host :

Rabbit

Isotype :

IgG

Size :

100μl

List Price :

330 USD

Storage Buffer :

1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Form :

Liquid

Storage Instruction :

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.

Purity :

Immunogen affinity purified.

Product type :

Rabbit polyclonal Antibody

Positive control :

Wild-type Raw264.7 whole cell lysate, HepG2 cell lysate, MCF-7 cell lysate, F9 cell lysate, A549 cell lysate, Hela, NIH/3T3, mouse kidney tissue, mouse stomach tissue, mouse brain tissue, human stomach carcinoma tissue, human tonsil tissue,

Molecular wt :

Predicted band size: 25 kDa

Subcellular location :

Nucleus, Cell membrane, Cytoplasm, Secreted.

Concentration :

1 mg/mL.

Recommended dilutions :

WB: 1:500-1:1,000;IF-Cell: 1:200;IHC-P: 1:200;FC: 1:50-1:100

Advanced Validation :

Knockdown (KD)

Pic img4 :

https://storage.huabio.cn/huabio/productImg/ER0913_4.jpg

Pic legend4 :

ICC staining HMGB1 in NIH/3T3 cells (green). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Pic img5 :

https://storage.huabio.cn/huabio/productImg/ER0913_5.jpg

Pic legend5 :

Immunohistochemical analysis of paraffin-embedded mouse kidney tissue using anti- HMGB1 antibody. Counter stained with hematoxylin.

Pic img6 :

https://storage.huabio.cn/huabio/productImg/ER0913_6.jpg

Pic legend6 :

Immunohistochemical analysis of paraffin-embedded mouse stomach tissue using anti- HMGB1 antibody. Counter stained with hematoxylin.

Pic img7 :

https://storage.huabio.cn/huabio/productImg/ER0913_7.jpg

Pic legend7 :

Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti- HMGB1 antibody. Counter stained with hematoxylin.

Pic img8 :

https://storage.huabio.cn/huabio/productImg/ER0913_8.jpg

Pic legend8 :

Immunohistochemical analysis of paraffin-embedded human stomach carcinoma tissue using anti- HMGB1 antibody. Counter stained with hematoxylin.

Pic img9 :

https://storage.huabio.cn/huabio/productImg/ER0913_9.jpg

Pic legend9 :

Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti- HMGB1 antibody. Counter stained with hematoxylin.

Pic img10 :

https://storage.huabio.cn/huabio/productImg/ER0913_10.jpg

Pic legend10 :

Flow cytometric analysis of Hela cells with HMGB1 antibody at 1/50 dilution (blue) compared with an unlabelled control (cells without incubation with primary antibody; red). Goat anti rabbit IgG (FITC) was used as the secondary antibody.

more info or order :

HUABIO product webpage