BAX | HUABIO ER0907 product information

product summary

company name :

HUABIO

product type :

antibody

product name :

BAX

catalog :

ER0907

quantity :

100μl

price :

330 USD

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

reactivity :

human, mouse, rat

application :

western blot, immunohistochemistry - paraffin section

more info or order :

HUABIO product webpage

image

image 1 :

Western blot analysis of BAX on different lysates with Rabbit anti-BAX antibody (ER0907) at 1/5,000 dilution and competitor's antibody at 1/1,000 dilution. Lane 1: HeLa cell lysate Lane 2: MCF7 cell lysate Lane 3: HEK-293 cell lysate Lysates/proteins at 15 µg/Lane. Predicted band size: 21 kDa Observed band size: 21 kDa Exposure time: 1 minute 34 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ER0907) at 1/5,000 dilution and competitor's antibody at 1/1,000 dilution were used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

image 2 :

Western blot analysis of BAX on different lysates with Rabbit anti-BAX antibody (ER0907) at 1/5,000 dilution. Lane 1: HeLa cell lysate Lane 2: MCF7 cell lysate Lane 3: Daudi cell lysate Lane 4: Raji cell lysate Lane 5: Jurkat cell lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 21 kDa Observed band size: 21 kDa Exposure time: 29 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ER0907) at 1/5,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:50,000 dilution was used for 1 hour at room temperature.

image 3 :

Western blot analysis of BAX on different lysates with Rabbit anti-BAX antibody (ER0907) at 1/20,000 dilution. Lane 1: MCF7-si NT cell lysate Lane 2: MCF7-si BAX cell lysate Lysates/proteins at 10 µg/Lane. Predicted band size: 21 kDa Observed band size: 21 kDa Exposure time: 3 minutes; ECL: K1802; 4-20% SDS-PAGE gel. ER0907 was shown to specifically react with BAX in MCF7-si NT cells. Weakened band was observed when MCF7-si BAX sample was tested. MCF7-si NT and MCF7-si BAX samples were subjected to SDS-PAGE. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM in TBST for 1 hour at room temperature. The primary antibody (ER0907, 1/20,000) and Loading control antibody (Rabbit anti-GAPDH, ET1601-4, 1/10,000) were used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-rabbit IgG-HRP Secondary Antibody (HA1001) at 1:50,000 dilution was used for 1 hour at room temperature.

product information

SKU :

ER0907

Target name :

BAX

Species reactivity :

Human,Mouse,Rat

Applications :

WB,IF-Cell,IHC-P

Conjugate :

Non-conjugated

Immunogen :

Synthetic peptide within Human BAX aa 1-50 / 192.

Uniprot id :

Q07812>SwissProt: Q07812 Human;SwissProt: Q07813 Mouse;SwissProt: Q63690 Rat

Host :

Rabbit

Isotype :

IgG

Size :

100μl

List Price :

330 USD

Storage Buffer :

1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Form :

Liquid

Storage Instruction :

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.

Purity :

Immunogen affinity purified.

Product type :

Rabbit polyclonal Antibody

Positive control :

HeLa cell lysate, MCF7 cell lysate, HEK-293 cell lysate, Daudi cell lysate, Raji cell lysate, Jurkat cell lysate, HepG2, F9, SHSY5Y, human colon carcinoma tissue, mouse kidney tissue.

Molecular wt :

Predicted band size: 21 kDa

Subcellular location :

Mitochondrion membrane, cytoplasm.

Concentration :

1 mg/mL.

Recommended dilutions :

WB: 1:5,000-1:20,000 ;IF-Cell: 1:200 ;IHC-P: 1:1,000

Advanced Validation :

Knockdown (KD)

Pic img4 :

https://storage.huabio.cn/huabio/productImg/ER0907_4.jpg

Pic legend4 :

Immunocytochemistry analysis of SHSY5Y cells labeling BAX with Rabbit anti-BAX antibody (ER0907) at 1/200 dilution. Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-BAX antibody (ER0907) at 1/200 dilution in 2% negative goat serum overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. Nuclear DNA was labelled in blue with DAPI. Beta Ⅲ tubulin (M1305-2, red) was stained at 1/200 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 647, HA1127) were used as the secondary antibody at 1/1,000 dilution.

Pic img5 :

https://storage.huabio.cn/huabio/productImg/ER0907_5.jpg

Pic legend5 :

ICC staining Bax in HepG2 cells (green). The nuclear counter stain is DAPI (blue).Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Pic img6 :

https://storage.huabio.cn/huabio/productImg/ER0907_6.jpg

Pic legend6 :

ICC staining Bax in F9 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Pic img7 :

https://storage.huabio.cn/huabio/productImg/ER0907_7.jpg

Pic legend7 :

Immunohistochemical analysis of paraffin-embedded human colon carcinoma tissue with Rabbit anti-BAX antibody (ER0907) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ER0907) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Pic img8 :

https://storage.huabio.cn/huabio/productImg/ER0907_8.jpg

Pic legend8 :

Immunohistochemical analysis of paraffin-embedded mouse kidney tissue with Rabbit anti-BAX antibody (ER0907) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ER0907) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Pic img9 :

https://storage.huabio.cn/huabio/productImg/ER0907_9.jpg

Pic legend9 :

Immunohistochemical analysis of paraffin-embedded rat kidney tissue with Rabbit anti-BAX antibody (ER0907) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ER0907) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

more info or order :

HUABIO product webpage