CD34 | HUABIO ER0802 product information

product summary

company name :

HUABIO

product type :

antibody

product name :

CD34

catalog :

ER0802

quantity :

100μl

price :

330 USD

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

reactivity :

human, mouse, rat

application :

western blot, flow cytometry, immunohistochemistry - paraffin section

more info or order :

HUABIO product webpage

image

image 1 :

Western blot analysis of CD34 on different lysates with Rabbit anti-CD34 antibody (ER0802) at 1/2,000 dilution. Lane 1: TF-1 cell lysate (15 µg/Lane) Lane 2: Human brain tissue lysate (20 µg/Lane) Lane 3: Mouse brain tissue lysate (20 µg/Lane) Lane 4: Rat brain tissue lysate (20 µg/Lane) Predicted band size: 41 kDa Observed band size: 100~120 kDa Exposure time: 30 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ER0802) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

image 2 :

Immunocytochemistry analysis of TF-1 cells labeling CD34 with Rabbit anti-CD34 antibody (ER0802) at 1/250dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-CD34 antibody (ER0802) at 1/250dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (M1305-2, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution.

image 3 :

Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-CD34 antibody. Counter stained with hematoxylin.

product information

SKU :

ER0802

Target name :

CD34

Species reactivity :

Human,Mouse,Rat,Zebrafish

Applications :

WB,IF-Cell,IHC-P,FC

Conjugate :

Non-conjugated

Immunogen :

Synthetic peptide within Human CD34 aa 336-385 / 385.

Uniprot id :

P28906>SwissProt: P28906 Human

Host :

Rabbit

Isotype :

IgG

Size :

100μl

List Price :

330 USD

Storage Buffer :

1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Form :

Liquid

Storage Instruction :

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.

Purity :

Immunogen affinity purified.

Product type :

Rabbit polyclonal Antibody

Positive control :

TF-1 cell lysate, human brain tissue lysate, mouse brain tissue lysate, rat brain tissue lysate, TF-1, human tonsil tissue, human liver carcinoma tissue.

Molecular wt :

Predicted band size: 41 kDa

Subcellular location :

Membrane

Concentration :

1 mg/mL.

Recommended dilutions :

WB: 1:1,000-1:2,000 ;IF-Cell: 1:250 ;IHC-P: 1:2,000-1:3,000 ;FC: 1:1,000

Pic img4 :

https://storage.huabio.cn/huabio/productImg/ER0802_4.jpg

Pic legend4 :

Immunohistochemical analysis of paraffin-embedded human liver carcinoma tissue using anti-CD34 antibody. Counter stained with hematoxylin.

Pic img5 :

https://storage.huabio.cn/huabio/productImg/ER0802_5.jpg

Pic legend5 :

Flow cytometric analysis of TF-1 cells labeling CD34. Cells were fixed and permeabilized. Then stained with the primary antibody (ER0802, 1/1,000) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).

more info or order :

HUABIO product webpage