Insulin | HUABIO EM80714 product information

product summary

company name :

HUABIO

product type :

antibody

product name :

Insulin

catalog :

EM80714

quantity :

100μl

price :

360.00 USD

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

A6-6

reactivity :

human, mouse, rat

application :

ELISA, immunohistochemistry - paraffin section, immunohistochemistry - frozen section

more info or order :

HUABIO product webpage

image

image 1 :

Immunofluorescence analysis of frozen mouse pancreas tissue with Mouse anti-Insulin antibody (EM80714) at 1/500 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for about 2 minutes in microwave oven. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS, and then probed with the primary antibody (EM80714, red) at 1/500 dilution overnight at 4 ℃, washed with PBS. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1125) was used as the secondary antibody at 1/1,000 dilution. Nuclei were counterstained with DAPI (blue). Glucagon (ET1702-20, green) was stained at 1/500 dilution overnight at +4℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution.

image 2 :

Immunofluorescence analysis of frozen mouse pancreas tissue with Mouse anti-Insulin antibody (EM80714) at 1/500 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for about 2 minutes in microwave oven. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS, and then probed with the primary antibody (EM80714, red) at 1/500 dilution overnight at 4 ℃, washed with PBS. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1125) was used as the secondary antibody at 1/1,000 dilution. Nuclei were counterstained with DAPI (blue). PPY (HA721515, green) was stained at 1/500 dilution overnight at +4℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution.

image 3 :

Immunofluorescence analysis of frozen rat pancreas tissue with Mouse anti-Insulin antibody (EM80714) at 1/500 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for about 2 minutes in microwave oven. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS, and then probed with the primary antibody (EM80714, red) at 1/500 dilution overnight at 4 ℃, washed with PBS. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1125) was used as the secondary antibody at 1/1,000 dilution. Nuclei were counterstained with DAPI (blue). PPY (HA721515, green) was stained at 1/500 dilution overnight at +4℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution.

product information

SKU :

EM80714

Target name :

Insulin

Species reactivity :

Human,Mouse,Rat

Applications :

IHC-P,ELISA,IF-Tissue,IHC-Fr

Conjugate :

Non-conjugated

Immunogen :

Synthetic peptide within human Insulin aa 25-54.

Uniprot id :

P01308>SwissProt: P01308 Human;SwissProt: P01325 Mouse;SwissProt: P01322 Rat

Host :

Mouse

Clone number :

A6-6

Isotype :

IgG1

Size :

100μl

List Price :

360.00 USD

Storage Buffer :

1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Form :

Liquid

Storage Instruction :

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.

Purity :

Protein A affinity purified.

Product type :

Mouse monoclonal Antibody

Positive control :

Human pancreas tissue, mouse pancreas tissue, rat pancreas tissue.

Molecular wt :

Predicted band size: 12 kDa

Subcellular location :

Secreted.

Concentration :

2 mg/mL.

Recommended dilutions :

IHC-P: 1:2,000 ;ELISA: 1:10,000 ;IF-Tissue: 1:200 ;IHC-Fr: 1:500

Pic img4 :

https://storage.huabio.cn/huabio/productImg/EM80714_4.jpg

Pic legend4 :

Immunohistochemical analysis of paraffin-embedded human pancreas tissue with Mouse anti-Insulin antibody (EM80714) at 1/2,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (EM80714) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Pic img5 :

https://storage.huabio.cn/huabio/productImg/EM80714_5.jpg

Pic legend5 :

Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue with Mouse anti-Insulin antibody (EM80714) at 1/2,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (EM80714) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Pic img6 :

https://storage.huabio.cn/huabio/productImg/EM80714_6.jpg

Pic legend6 :

Immunohistochemical analysis of paraffin-embedded rat pancreas tissue with Mouse anti-Insulin antibody (EM80714) at 1/2,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (EM80714) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Pic img7 :

https://storage.huabio.cn/huabio/productImg/EM80714_7.jpg

Pic legend7 :

Immunofluorescence analysis of paraffin-embedded mouse pancreas tissue labeling Insulin with Mouse anti-Insulin antibody (EM80714) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS, and then probed with the primary antibody (EM80714, green) at 1/200 dilution overnight at 4 ℃, washed with PBS. Goat Anti-Mouse IgG H&L (iFluor™ 488, HA1125) was used as the secondary antibody at 1/1,000 dilution. Nuclei were counterstained with DAPI (blue).

more info or order :

HUABIO product webpage