Caveolin-1 | HUABIO EM40728 product information

product summary

company name :

HUABIO

product type :

antibody

product name :

Caveolin-1

catalog :

EM40728

quantity :

100μl

price :

360.00 USD

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

19-D6

reactivity :

human, mouse, rat

application :

western blot, immunohistochemistry - paraffin section

more info or order :

HUABIO product webpage

image

image 1 :

Western blot analysis of Caveolin-1 on different lysates with Mouse anti-Caveolin-1 antibody (EM40728) at 1/500 dilution. Lane 1: A431 cell lysate (20 µg/Lane) Lane 2: NIH/3T3 cell lysate (20 µg/Lane) Lane 3: C6 cell lysate (20 µg/Lane) Lane 4: Rat lung tissue lysate (30 µg/Lane) Predicted band size: 20 kDa Observed band size: 20 kDa Exposure time: Lane 1: 3 minutes; Lane 2-4: 6 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (EM40728) at 1/500 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1/50,000 dilution was used for 1 hour at room temperature.

image 2 :

Immunohistochemical analysis of paraffin-embedded mouse bladder tissue with Mouse anti-Caveolin-1 antibody (EM40728) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (EM40728) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

image 3 :

Immunohistochemical analysis of paraffin-embedded rat lung tissue with Mouse anti-Caveolin-1 antibody (EM40728) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (EM40728) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

product information

SKU :

EM40728

Target name :

Caveolin-1

Species reactivity :

Human,Mouse,Rat

Applications :

WB,IHC-P

Conjugate :

Non-conjugated

Immunogen :

Synthetic peptide within Mouse Caveolin-1 aa 129-178 / 178.

Uniprot id :

Q03135>SwissProt: Q03135 Human;SwissProt: P49817 Mouse;SwissProt: P41350 Rat

Host :

Mouse

Clone number :

19-D6

Isotype :

IgG1

Size :

100μl

List Price :

360.00 USD

Storage Buffer :

1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Form :

Liquid

Storage Instruction :

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.

Purity :

Protein A affinity purified.

Product type :

Mouse monoclonal Antibody

Positive control :

A431 cell lysate, NIH/3T3 cell lysate, C6 cell lysate, Rat lung tissue lysate, mouse bladder tissue, rat lung tissue, human lung tissue, mouse lung tissue.

Molecular wt :

Predicted band size: 20 kDa

Subcellular location :

Cell membrane, Golgi apparatus membrane.

Concentration :

2 mg/mL.

Recommended dilutions :

WB: 1:500 ;IHC-P: 1:200-1:1,000

Pic img4 :

https://storage.huabio.cn/huabio/productImg/EM40728_4.jpg

Pic legend4 :

Immunohistochemical analysis of paraffin-embedded human lung tissue using anti-Caveolin-1 antibody. Counter stained with hematoxylin.

Pic img5 :

https://storage.huabio.cn/huabio/productImg/EM40728_5.jpg

Pic legend5 :

Immunohistochemical analysis of paraffin-embedded mouse lung tissue using anti-Caveolin-1 antibody. Counter stained with hematoxylin.

more info or order :

HUABIO product webpage