IDH1 | HUABIO EM40705 product information

product summary

company name :

HUABIO

product type :

antibody

product name :

IDH1

catalog :

EM40705

quantity :

100μl

price :

360.00 USD

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

A11-B4

reactivity :

human, mouse, rat, zebrafish

application :

western blot, immunohistochemistry - paraffin section

more info or order :

HUABIO product webpage

image

image 1 :

Western blot analysis of IDH1 on different lysates with Mouse anti-IDH1 antibody (EM40705) at 1/2,000 dilution. Lane 1: HepG2 cell lysate Lane 2: HeLa cell lysate Lane 3: HEK-293 cell lysate Lane 4: A431 cell lysate Lane 5: Neuro-2a cell lysate Lane 6: PC-12 cell lysate Lane 7: COS-1 cell lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 47 kDa Observed band size: 42 kDa Exposure time: 15 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (EM40705) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1/50,000 dilution was used for 1 hour at room temperature.

image 2 :

Immunohistochemical analysis of paraffin-embedded human colon carcinoma tissue using anti-IDH1 antibody. Counter stained with hematoxylin.

image 3 :

Immunohistochemical analysis of paraffin-embedded human breast tissue using anti-IDH1 antibody. Counter stained with hematoxylin.

product information

SKU :

EM40705

Target name :

IDH1

Species reactivity :

Human,Mouse,Rat,Monkey,Zebrafish

Applications :

WB,IHC-P

Conjugate :

Non-conjugated

Immunogen :

Synthetic peptide within Human IDH1 aa 101-150 / 414.

Uniprot id :

O75874>SwissProt: O75874 Human;SwissProt: O88844 Mouse;SwissProt: P41562 Rat

Host :

Mouse

Clone number :

A11-B4

Isotype :

IgG1

Size :

100μl

List Price :

360.00 USD

Storage Buffer :

1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Form :

Liquid

Storage Instruction :

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.

Purity :

Protein A affinity purified.

Product type :

Mouse monoclonal Antibody

Positive control :

HepG2 cell lysate, HeLa cell lysate, HEK-293 cell lysate, A431 cell lysate, Neuro-2a cell lysate, PC-12 cell lysate, COS-1 cell lysate, human colon carcinoma tissue, human breast tissue, human kidney tissue.

Molecular wt :

Predicted band size: 47 kDa

Subcellular location :

Cytoplasm

Concentration :

2 mg/mL.

Recommended dilutions :

WB: 1:2,000 ;IHC-P: 1:100-1:200

Pic img4 :

https://storage.huabio.cn/huabio/productImg/EM40705_4.jpg

Pic legend4 :

Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-IDH1 antibody. Counter stained with hematoxylin.

more info or order :

HUABIO product webpage