p38 alpha / MAPK14 | HUABIO EM40307 product information

product summary

company name :

HUABIO

product type :

antibody

product name :

p38 alpha / MAPK14

catalog :

EM40307

quantity :

100μl

price :

360.00 USD

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

9-B9

reactivity :

human, mouse, rat

application :

western blot, flow cytometry, immunohistochemistry - paraffin section

more info or order :

HUABIO product webpage

image

image 1 :

Western blot analysis of p38 alpha / MAPK14 on different lysates with Mouse anti-p38 alpha / MAPK14 antibody (EM40307) at 1/1,000 dilution. Lane 1: HeLa cell lysate Lane 2: Jurkat cell lysate Lane 3: RAW264.7 cell lysate Lane 4: PC-12 cell lysate Lane 5: C6 cell lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 41 kDa Observed band size: 38 kDa Exposure time: 10 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (EM40307) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1/50,000 dilution was used for 1 hour at room temperature.

image 2 :

Immunocytochemistry analysis of HeLa cells untreated / treated with UV for 30 minutes then recover 30 minutes labeling p38 alpha / MAPK14 with Mouse anti-p38 alpha / MAPK14 antibody (EM40307) at 1/500 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Mouse anti-p38 alpha / MAPK14 antibody (EM40307) at 1/500 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Mouse IgG H&L (iFluor™ 488, HA1125) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. beta Tubulin (ET1602-4, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Rabbit IgG H&L (iFluor™ 594, HA1122) were used as the secondary antibody at 1/1,000 dilution.

image 3 :

Immunohistochemical analysis of paraffin-embedded mouse stomach tissue with Mouse anti-p38 alpha / MAPK14 antibody (EM40307) at 1/5,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (EM40307) at 1/5,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

product information

SKU :

EM40307

Target name :

p38 alpha / MAPK14

Species reactivity :

Human,Mouse,Rat

Applications :

WB,IF-Cell,IHC-P,FC

Conjugate :

Non-conjugated

Immunogen :

Synthetic peptide within Human MAPK14 aa 1-50 / 360.

Uniprot id :

Q16539>SwissProt: Q16539 Human;SwissProt: P47811 Mouse;SwissProt: P70618 Rat

Host :

Mouse

Clone number :

9-B9

Isotype :

IgG2a

Size :

100μl

List Price :

360.00 USD

Storage Buffer :

1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Form :

Liquid

Storage Instruction :

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.

Purity :

Protein A affinity purified.

Product type :

Mouse monoclonal Antibody

Positive control :

HeLa cell lysate, Jurkat cell lysate, RAW264.7 cell lysate, PC-12 cell lysate, C6 cell lysate, HeLa cells treated with UV for 30 minutes then recover 30 minutes, mouse stomach tissue, rat stomach tissue, HeLa.

Molecular wt :

Predicted band size: 41 kDa

Subcellular location :

Cytoplasm, nucleus

Concentration :

2 mg/mL.

Recommended dilutions :

WB: 1:1,000 ;IF-Cell: 1:500 ;IHC-P: 1:5,000 ;FC: 1:1,000

Pic img4 :

https://storage.huabio.cn/huabio/productImg/EM40307_4.jpg

Pic legend4 :

Immunohistochemical analysis of paraffin-embedded rat stomach tissue with Mouse anti-p38 alpha / MAPK14 antibody (EM40307) at 1/5,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (EM40307) at 1/5,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Pic img5 :

https://storage.huabio.cn/huabio/productImg/EM40307_5.jpg

Pic legend5 :

Flow cytometric analysis of HeLa cells labeling p38 alpha / MAPK14. Cells were fixed and permeabilized. Then stained with the primary antibody (EM40307, 1/1,000) (red) compared with Mouse IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Mouse IgG Secondary antibody (HA1125) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).

more info or order :

HUABIO product webpage