Myc tag | HUABIO EM31105 product information

product summary

company name :

HUABIO

product type :

antibody

product name :

Myc tag

catalog :

EM31105

quantity :

100μl

price :

360.00 USD

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

A3-B4

application :

western blot, ELISA, immunoprecipitation, flow cytometry

more info or order :

HUABIO product webpage

image

image 1 :

Western blot analysis of Myc tag on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (EM31105, 1/2,000) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1:5,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: C-terminal Myc-tagged recombinant protein Lane 2: N-terminal Myc-tagged recombinant protein

image 2 :

ICC staining of Myc tag in Hela cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (EM31105, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 Goat anti-Mouse IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).

image 3 :

Myc tag was immunoprecipitated in 2µg C terminal Myc Tag fusion protein lysate with EM31105 at 2 µg/20 µl agarose. Western blot was performed from the immunoprecipitate using R1208-1 at 1/1,000 dilution. Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 60 mins at room temperature. Lane 1: Myc Tag fusion protein lysate (input). Lane 2: EM1902-38 IP in Myc Tag fusion protein lysate. Lane 3: EM31105 IP in Myc Tag fusion protein lysate. Lane 4: Mouse IgG instead of EM31105 in Myc Tag fusion protein lysate. Blocking/Dilution buffer: 5% NFDM/TBST

product information

SKU :

EM31105

Target name :

Myc tag

Species reactivity :

Species independent

Applications :

WB,IF-Cell,ELISA,FC,IP

Conjugate :

Non-conjugated

Immunogen :

Synthetic peptide immune sequence is EQKLISEEDLC-KLH.

Uniprot id :

Host :

Mouse

Clone number :

A3-B4

Isotype :

IgG2b

Size :

100μl

List Price :

360.00 USD

Storage Buffer :

1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Form :

Liquid

Storage Instruction :

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.

Purity :

Protein G affinity purified.

Product type :

Mouse monoclonal Antibody

Positive control :

Myc-tagged recombinant protein.

Concentration :

2 mg/mL.

Recommended dilutions :

WB: 1:2,000 ;IF-Cell: 1:50 ;FC: 1:50-1:100 ;IP: 2-5 µg/ml.

Pic img4 :

https://storage.huabio.cn/huabio/productImg/EM31105_4.jpg

Pic legend4 :

Myc tag was immunoprecipitated in 2µg N terminal Myc Tag fusion protein lysate with EM31105 at 2 µg/20 µl agarose. Western blot was performed from the immunoprecipitate using R1208-1 at 1/1,000 dilution. Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 60 mins at room temperature. Lane 1: Myc Tag fusion protein lysate (input). Lane 2: EM1902-38 IP in Myc Tag fusion protein lysate. Lane 3: EM31105 IP in Myc Tag fusion protein lysate. Lane 4: Mouse IgG instead of EM31105 in Myc Tag fusion protein lysate. Blocking/Dilution buffer: 5% NFDM/TBST

more info or order :

HUABIO product webpage