product summary
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company name :
HUABIO
product type :
antibody
product name :
GFP
catalog :
EM30501
quantity :
100μl
price :
360.00 USD
clonality :
monoclonal
host :
mouse
conjugate :
nonconjugated
clone name :
2-D8
application :
western blot, ELISA, immunoprecipitation, flow cytometry
more info or order :
image
image 1 :
Western blot analysis of GFP on transfected K562 with GFP using anti-GFP antibody at 1/2000 dilution.
image 2 :
ICC staining GFP in transfected K562 cells (green). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
image 3 :
GFP tag was immunoprecipitated in 5µg GFP Tag fusion protein lysate with EM30501 at 2 µg/20 µl agarose. Western blot was performed from the immunoprecipitate using ET1604-25 at 1/1000 dilution. Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 60 mins at room temperature.
Lane 1: GFP Tag fusion protein lysate (input).
Lane 2: M1210-1 IP in GFP Tag fusion protein lysate.
Lane 3: EM30501 IP in GFP Tag fusion protein lysate.
Lane 4: Mouse IgG instead of EM30501 in GFP Tag fusion protein lysate.
Blocking/Dilution buffer: 5% NFDM/TBST
product information
SKU :
EM30501
Target name :
GFP
Species reactivity :
Species independent
Applications :
WB,IF-Cell,ELISA,FC,IP
Conjugate :
Non-conjugated
Immunogen :
Recombinant full length protein of Aequorea victoria GFP.
Uniprot id :
P42212>SwissProt: P42212 Aequorea victoria
Host :
Mouse
Clone number :
2-D8
Isotype :
IgG1
Size :
100μl
List Price :
360.00 USD
Storage Buffer :
1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Form :
Liquid
Storage Instruction :
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
Purity :
Protein A affinity purified.
Product type :
Mouse monoclonal Antibody
Positive control :
Transfected K562 with GFP.
Molecular wt :
Predicted band size: 27 kDa
Concentration :
2 mg/mL.
Recommended dilutions :
WB: 1:2,000
;IF-Cell: 1:200
;IP: 2-5 µg/ml.
more info or order :
company information
HUABIO
Founded in 2007, HUABIO is dedicated to developing high-quality antibodies that advance innovation. We are passionate about the accuracy, efficiency, and consistency of our products. That is why we have invested in new production platforms, like recombinant rabbit monoclonals, alpaca nanobodies, and adopted aggressive QA standards to deliver cutting-edge antibodies with uncompromised quality.
We hope to see you at your next discovery!
We hope to see you at your next discovery!
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