beta Actin | HUABIO EM21002 product information

product summary

company name :

HUABIO

product type :

antibody

product name :

beta Actin

catalog :

EM21002

quantity :

100μl

price :

360.00 USD

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

A2-F6

reactivity :

human, mouse, rat

application :

western blot, flow cytometry, immunohistochemistry - paraffin section

more info or order :

HUABIO product webpage

image

image 1 :

Western blot analysis of beta Actin on Hela cell lysates with Mouse anti-beta Actin antibody (EM21002). Hela cell lysates at 10 µg/Lane. Predicted band size: 42 kDa Observed band size: 42 kDa 12% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (EM21002) at serial dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1:100,000 dilution was used for 1 hour at room temperature.

image 2 :

Western blot analysis of beta Actin on different lysates with Mouse anti-beta Actin antibody (EM21002) at 1/40,000 dilution. Cell lysates at 10 µg/Lane, tissue lysates at 20 µg/Lane. Predicted band size: 42 kDa Observed band size: 42 kDa Exposure time: 1 minute; 12% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (EM21002) at 1/40,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1:100,000 dilution was used for 1 hour at room temperature.

image 3 :

Western blot analysis of beta Actin on different lysates with Mouse anti-beta Actin antibody (EM21002) at 1/40,000 dilution. Lane 1: THP-1 cell lysate Lane 2: HepG2 cell lysate Lane 3: PMVEC cell lysate Lane 4: NIH/3T3 cell lysate Lane 5: L929 cell lysate Lysates/proteins at 10 µg/Lane. Predicted band size: 42 kDa Observed band size: 42 kDa Exposure time: 2 minutes; 12% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (EM21002) at 1/40,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1/50,000 dilution was used for 1 hour at room temperature.

product information

SKU :

EM21002

Target name :

beta Actin

Species reactivity :

Human,Mouse,Rat

Applications :

WB,IF-Cell,IHC-P,FC

Conjugate :

Non-conjugated

Immunogen :

Synthetic peptide (KLH-coupled) within human Beta-actin aa 1-50.

Uniprot id :

P60709>SwissProt: P60709 Human;SwissProt: P60710 Mouse;SwissProt: P60711 Rat

Host :

Mouse

Clone number :

A2-F6

Isotype :

IgG1

Size :

100μl

List Price :

360.00 USD

Storage Buffer :

1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Form :

Liquid

Storage Instruction :

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.

Purity :

Protein A affinity purified.

Product type :

Mouse monoclonal Antibody

Positive control :

Hela cell lysate, THP-1 cell lysate, HepG2 cell lysate, MCF-7 cell lysate, PC-12 cell lysate, PMVEC cell lysate, RH-35 cell lysate, L6 cell lysate, NIH/3T3 cell lysate, L929 cell lysate, RAW264.7 cell lysate, B16F1 cell lysate, MEF cell lysate, Hela, A549, NIH/3T3, human colon carcinoma tissue, mouse prostate tissue, mouse kidney tissue.

Molecular wt :

Predicted band size: 42 kDa

Subcellular location :

Cytoplasm

Concentration :

2 mg/mL.

Recommended dilutions :

WB: 1:40,000-100,000 ;IF-Cell: 1:100-1:200 ;IHC-P: 1:1,000 ;FC: 1:1,000

Pic img4 :

https://storage.huabio.cn/huabio/productImg/EM21002_4.jpg

Pic legend4 :

Immunocytochemistry analysis of HeLa cells labeling beta Actin with Mouse anti-beta Actin antibody (EM21002) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Mouse anti-beta Actin antibody (EM21002) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Mouse IgG H&L (iFluor™ 488, HA1125) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. beta Tubulin (ET1602-4, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Rabbit IgG H&L (iFluor™ 594, HA1122) was used as the secondary antibody at 1/1,000 dilution.

Pic img5 :

https://storage.huabio.cn/huabio/productImg/EM21002_5.jpg

Pic legend5 :

Immunohistochemical analysis of paraffin-embedded mouse lung tissue with Mouse anti-beta Actin antibody (EM21002) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (EM21002) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Pic img6 :

https://storage.huabio.cn/huabio/productImg/EM21002_6.jpg

Pic legend6 :

Immunohistochemical analysis of paraffin-embedded rat lung tissue with Mouse anti-beta Actin antibody (EM21002) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (EM21002) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

more info or order :

HUABIO product webpage