Bmi1 | HUABIO EM20602 product information

product summary

company name :

HUABIO

product type :

antibody

product name :

Bmi1

catalog :

EM20602

quantity :

100μl

price :

360.00 USD

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

B3-G5

reactivity :

human, mouse, rat

application :

western blot, flow cytometry, immunohistochemistry - paraffin section

more info or order :

HUABIO product webpage

image

image 1 :

Western blot analysis of Bmi1 on different lysates using anti-Bmi1 antibody at 1/1,000 dilution. Positive control: Lane 1: 293T Lane 2: Jurkat Lane 3: Hela Lane 4: MCF-7 Lane 5: HepG2 Lane 6: NIH/3T3 Lane 7: PC12 Lane 8: Mouse kidney Lane 9: Human kidney Lane 10: K562 Lane 11: Human brain

image 2 :

Immunocytochemistry analysis of LOVO cells labeling Bmi1 with Mouse anti-Bmi1 antibody (EM20602) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 30 minutes, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes, and then blocked with 2% BSA for 30 minutes at room temperature. Cells were then incubated with Mouse anti-Bmi1 antibody (EM20602) at 1/50 dilution in 2% BSA overnight at 4 ℃. Goat Anti-Mouse IgG H&L (iFluor™ 488, HA1125) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.

image 3 :

Immunocytochemistry analysis of HeLa cells labeling Bmi1 with Mouse anti-Bmi1 antibody (EM20602) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 30 minutes, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes, and then blocked with 2% BSA for 30 minutes at room temperature. Cells were then incubated with Mouse anti-Bmi1 antibody (EM20602) at 1/50 dilution in 2% BSA overnight at 4 ℃. Goat Anti-Mouse IgG H&L (iFluor™ 488, HA1125) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.

product information

SKU :

EM20602

Target name :

Bmi1

Species reactivity :

Human,Mouse,Rat

Applications :

WB,IF-Cell,IHC-P,FC

Conjugate :

Non-conjugated

Immunogen :

Recombinant protein within human Bmi1 full sequence.

Uniprot id :

P35226>SwissProt: P35226 Human;SwissProt: P25916 Mouse;Entrez Gene: 307151 Rat

Host :

Mouse

Clone number :

B3-G5

Isotype :

IgG2a

Size :

100μl

List Price :

360.00 USD

Storage Buffer :

1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Form :

Liquid

Storage Instruction :

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.

Purity :

Protein A affinity purified.

Product type :

Mouse monoclonal Antibody

Positive control :

Hela, HepG2, Jurkat, 293T, MCF-7, PC12, K562, NIH/3T3, LOVO, human tonsil tissue, human colon cancer tissue, human breast cancer tissue, mouse kidney tissue, human kidney tissue, human brain tissue.

Molecular wt :

Predicted band size: 37 kDa

Subcellular location :

Nucleus, Cytoplasm

Concentration :

2 mg/mL.

Recommended dilutions :

WB: 1:1,000 ;IHC-P: 1:200 ;IF-Cell: 1:50-1:100 ;FC: 1:100-1:200

Pic img4 :

https://storage.huabio.cn/huabio/productImg/EM20602_4.jpg

Pic legend4 :

Immunocytochemistry analysis of NIH/3T3 cells labeling Bmi1 with Mouse anti-Bmi1 antibody (EM20602) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Mouse anti-Bmi1 antibody (EM20602) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Mouse IgG H&L (iFluor™ 488, HA1125) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. beta Tubulin (ET1602-4, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Rabbit IgG H&L (iFluor™ 594, HA1122) was used as the secondary antibody at 1/1,000 dilution.

Pic img5 :

https://storage.huabio.cn/huabio/productImg/EM20602_5.jpg

Pic legend5 :

Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-Bmi1 antibody. Counter stained with hematoxylin.

Pic img6 :

https://storage.huabio.cn/huabio/productImg/EM20602_6.jpg

Pic legend6 :

Immunohistochemical analysis of paraffin-embedded human colon cancer tissue using anti-Bmi1 antibody. Counter stained with hematoxylin.

Pic img7 :

https://storage.huabio.cn/huabio/productImg/EM20602_7.jpg

Pic legend7 :

Immunohistochemical analysis of paraffin-embedded human breast cancer tissue using anti-BMI1 antibody. Counter stained with hematoxylin.

Pic img8 :

https://storage.huabio.cn/huabio/productImg/EM20602_8.jpg

Pic legend8 :

Immunohistochemical analysis of paraffin-embedded mouse kidney tissue with Mouse anti-Bmi1 antibody (EM20602) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (EM20602) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Pic img9 :

https://storage.huabio.cn/huabio/productImg/EM20602_9.jpg

Pic legend9 :

Immunohistochemical analysis of paraffin-embedded rat kidney tissue with Mouse anti-Bmi1 antibody (EM20602) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (EM20602) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Pic img10 :

https://storage.huabio.cn/huabio/productImg/EM20602_10.jpg

Pic legend10 :

Flow cytometric analysis of Hela cells with BMI1 antibody at 1/100 dilution (blue) compared with an unlabelled control (cells without incubation with primary antibody; red). Goat anti mouse IgG (FITC) was used as the secondary antibody.

more info or order :

HUABIO product webpage