CD55 | HUABIO EM1902-42 product information

product summary

company name :

HUABIO

product type :

antibody

product name :

CD55

catalog :

EM1902-42

quantity :

100μl

price :

360.00 USD

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

12F1

reactivity :

human

application :

western blot, flow cytometry

more info or order :

HUABIO product webpage

image

image 1 :

Western blot analysis of CD55 on different lysates with Mouse anti-CD55 antibody (EM1902-42) at 1/500 dilution. Lane 1: A549 cell lysate Lane 2: LNCaP cell lysate (negative) Lane 3: HeLa cell lysate Lane 4: MDA-MB-231 cell lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 41 kDa Observed band size: 70-75 kDa Exposure time: 1 minute 2 seconds; ECL: K1802; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (EM1902-42) at 1/500 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1/50,000 dilution was used for 1 hour at room temperature.

image 2 :

Flow cytometric analysis of CD55 was done on SW1990 cells. The cells were fixed, permeabilized and stained with the primary antibody (EM1902-42, 1/50) (red). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated Goat anti-Mouse IgG Secondary antibody at 1/1000 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; black).

product information

SKU :

EM1902-42

Target name :

CD55

Species reactivity :

Human

Applications :

WB,FC

Conjugate :

Non-conjugated

Immunogen :

Recombinant protein within human CD55 aa 150- 381.

Uniprot id :

P08174>SwissProt: P08174 Human

Host :

Mouse

Clone number :

12F1

Isotype :

IgG1

Size :

100μl

List Price :

360.00 USD

Storage Buffer :

1*TBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.

Form :

Liquid

Storage Instruction :

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.

Purity :

Protein G affinity purified.

Product type :

Mouse monoclonal Antibody

Positive control :

A549 cell lysate, HeLa cell lysate, MDA-MB-231 cell lysate, SW1990.

Molecular wt :

Predicted band size: 41 kDa.

Subcellular location :

Cell membrane, Membrane, Secreted.

Concentration :

2 mg/mL.

Recommended dilutions :

WB: 1:500-1:1,000 ;FC: 1:50-1:100

Advanced Validation :

Relative expression (RE)

more info or order :

HUABIO product webpage