CELF2 | HUABIO EM1902-41 product information

product summary

company name :

HUABIO

product type :

antibody

product name :

CELF2

catalog :

EM1902-41

quantity :

100μl

price :

360.00 USD

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

D7-B5

reactivity :

human, mouse, rat

application :

western blot, immunohistochemistry - paraffin section

more info or order :

HUABIO product webpage

image

image 1 :

Western blot analysis of CELF2 on different lysates with Rabbit anti-CELF2 antibody (EM1902-41) at 1/1,000 dilution. Lane 1: PC-3M cell lysate (20 µg/Lane) Lane 2: Jurkat cell lysate (20 µg/Lane) Lane 3: THP-1 cell lysate (20 µg/Lane) Lane 4: Mouse colon tissue lysate (40 µg/Lane) Lane 5: Rat skeletal muscle tissue lysate (40 µg/Lane) Predicted band size: 54 kDa Observed band size: 48 kDa Exposure time: 14 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (EM1902-41) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

image 2 :

Immunohistochemical analysis of paraffin-embedded rat brain tissue using anti-CELF2 antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (EM1902-41, 1/400) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

image 3 :

Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti-CELF2 antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (EM1902-41, 1/400) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

product information

SKU :

EM1902-41

Target name :

CELF2

Species reactivity :

Human,Mouse,Rat

Applications :

WB,IHC-P

Conjugate :

Non-conjugated

Immunogen :

Synthetic peptide within Human CELF2 aa 450-508.

Uniprot id :

O95319>SwissProt: O95319 Human;SwissProt: Q9Z0H4 Mouse;SwissProt: Q792H5 Rat

Host :

Mouse

Clone number :

D7-B5

Isotype :

IgG1

Size :

100μl

List Price :

360.00 USD

Storage Buffer :

1*TBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.

Form :

Liquid

Storage Instruction :

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.

Purity :

Protein G affinity purified.

Product type :

Mouse monoclonal Antibody

Positive control :

PC-3M cell lysate, Jurkat cell lysate, THP-1 cell lysate, mouse colon tissue lysate, rat skeletal muscle tissue lysate, rat brain tissue, mouse brain tissue.

Molecular wt :

Predicted band size: 54 kDa

Subcellular location :

Cytoplasm, Nucleus.

Concentration :

2 mg/mL.

Recommended dilutions :

WB: 1:1,000 ;IHC-P: 1:100-1:500

more info or order :

HUABIO product webpage