Glutamine Synthetase | HUABIO EM1902-39 product information

product summary

company name :

HUABIO

product type :

antibody

product name :

Glutamine Synthetase

catalog :

EM1902-39

quantity :

100μl

price :

360.00 USD

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

A3G2

reactivity :

human, mouse, rat

application :

western blot, flow cytometry, immunohistochemistry - paraffin section

more info or order :

HUABIO product webpage

image

image 1 :

Western blot analysis of Glutamine Synthetase on different lysates with Mouse anti-Glutamine Synthetase antibody (EM1902-39) at 1/2,000 dilution. Lane 1: HeLa cell lysate Lane 2: MCF7 cell lysate Lane 3: HepG2 cell lysate Lane 4: K-562 cell lysate Lane 5: Jurkat cell lysate Lane 6: HEK-293 cell lysate Lane 7: SK-Br-3 cell lysate Lane 8: Human liver tissue lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 42 kDa Observed band size: 42 kDa Exposure time: 30 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (EM1902-39) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1/50,000 dilution was used for 1 hour at room temperature.

image 2 :

Fluorescence multiplex immunohistochemical analysis of mouse liver (Formalin/PFA-fixed paraffin-embedded sections). Panel A: the merged image of anti-Desmin (ET1606-30, White), anti-HNF4α (HA721006, Red) and anti-GS (EM1902-39, Yellow) on liver. HRP Conjugated UltraPolymer Goat Polyclonal Antibody HA1119/HA1120 was used as a secondary antibody. The immunostaining was performed with the Sequential Immuno-staining Kit (IRISKit™MH010101, www.luminiris.cn). The section was incubated in three rounds of staining: in the order of ET1606-30 (1/800 dilution), HA721006 (1/5,000 dilution) and EM1902-39 (1/2,000 dilution) for 20 mins at room temperature. Each round was followed by a separate fluorescent tyramide signal amplification system. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 30 mins at 95℃. DAPI (blue) was used as a nuclear counter stain. Image acquisition was performed with Zeiss Observer 7 Inverted Fluorescence Microscope.

image 3 :

Fluorescence multiplex immunohistochemical analysis of mouse liver (Formalin/PFA-fixed paraffin-embedded sections). Panel A: the merged image of anti-β-catenin (ET1601-5, Tangerine), anti-αSMA (ET1607-53, Yellow), anti-SOX9 (ET1611-56, Green), anti-Albumin (ET1702-55, Cyan) anti-GS (EM1902-39, Magenta) and anti-CK19 (ET1601-6, Orange) on liver. HRP Conjugated UltraPolymer Goat Polyclonal Antibody HA1119/HA1120 was used as a secondary antibody. The immunostaining was performed with the Sequential Immuno-staining Kit (IRISKit™MH010101, www.luminiris.cn). The section was incubated in six rounds of staining: in the order of ET1601-5 (1/2,000 dilution), ET1607-53 (1/3,000 dilution), ET1611-56 (1/1,500 dilution), ET1702-55 (1/3,000 dilution), EM1902-39 (1/2,000 dilution) and ET1601-6 (1/3,000 dilution) for 20 mins at room temperature. Each round was followed by a separate fluorescent tyramide signal amplification system. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 30 mins at 95℃. DAPI (blue) was used as a nuclear counter stain. Image acquisition was performed with Olympus VS200 Slide Scanner.

product information

SKU :

EM1902-39

Target name :

Glutamine Synthetase

Species reactivity :

Human,Mouse,Rat

Applications :

WB,IHC-P,FC,mIHC,IF-Tissue

Conjugate :

Non-conjugated

Immunogen :

Recombinant protein within human Glutamine synthetase aa 190-373.

Uniprot id :

P15104>SwissProt: P15104 Human;SwissProt: P15105 Mouse;SwissProt: P09606 Rat

Host :

Mouse

Clone number :

A3G2

Isotype :

IgG2a

Size :

100μl

List Price :

360.00 USD

Storage Buffer :

1*TBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.

Form :

Liquid

Storage Instruction :

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.

Purity :

Protein G affinity purified.

Product type :

Mouse monoclonal Antibody

Positive control :

HeLa cell lysate, MCF7 cell lysate, HepG2 cell lysate, K-562 cell lysate, Jurkat cell lysate, HEK-293 cell lysate, SK-Br-3 cell lysate, human liver tissue lysate, human spleen tissue, mouse liver tissue, rat liver tissue, THP-1.

Molecular wt :

Predicted band size: 42 kDa

Subcellular location :

Microsome, Cytosol, Mitochondrion, Cell membrane.

Concentration :

2 mg/mL.

Recommended dilutions :

WB: 1:500-1:2,000 ;IHC-P: 1:500-1:2,000 ;FC: 1:50-1:100 ;mIHC: 1:2,000 ;IF-Tissue: 1:200-1:500

Pic img4 :

https://storage.huabio.cn/huabio/productImg/EM1902-39_4.jpg

Pic legend4 :

Immunohistochemical analysis of paraffin-embedded human spleen tissue with Mouse anti-Glutamine Synthetase antibody (EM1902-39) at 1/2,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (EM1902-39) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Pic img5 :

https://storage.huabio.cn/huabio/productImg/EM1902-39_5.jpg

Pic legend5 :

Immunohistochemical analysis of paraffin-embedded mouse liver tissue with Mouse anti-Glutamine Synthetase antibody (EM1902-39) at 1/2,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (EM1902-39) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Pic img6 :

https://storage.huabio.cn/huabio/productImg/EM1902-39_6.jpg

Pic legend6 :

Immunohistochemical analysis of paraffin-embedded rat liver tissue with Mouse anti-Glutamine Synthetase antibody (EM1902-39) at 1/500 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (EM1902-39) at 1/500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Pic img7 :

https://storage.huabio.cn/huabio/productImg/EM1902-39_7.jpg

Pic legend7 :

Flow cytometric analysis of Glutamine Synthetase was done on THP-1 cells. The cells were fixed, permeabilized and stained with the primary antibody (EM1902-39, 1/50) (red). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated Goat anti-Mouse IgG Secondary antibody at 1/1000 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; black).

more info or order :

HUABIO product webpage