Myc tag | HUABIO EM1902-38 product information

product summary

company name :

HUABIO

product type :

antibody

product name :

Myc tag

catalog :

EM1902-38

quantity :

100μl

price :

360.00 USD

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

A3C8

application :

western blot, ELISA, immunoprecipitation

more info or order :

HUABIO product webpage

image

image 1 :

Western blot analysis of Myc tag on C-terminal Myc-tagged recombinant protein lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (EM1902-38, 1/100,000) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1:10,000 dilution was used for 1 hour at room temperature.

image 2 :

Western blot analysis of Myc tag on N-terminal Myc-tagged recombinant protein lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (EM1902-38, 1/100,000) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1:10,000 dilution was used for 1 hour at room temperature.

image 3 :

Immobilized N-terminal Myc-tagged recombinant protein. at 1 μg/ml overnight at 4℃. Then blocked with 1% BSA for 1 hour at 37℃, and incubated with the primary antibody for 1 hour at 25℃.

product information

SKU :

EM1902-38

Target name :

Myc tag

Species reactivity :

Species independent

Applications :

WB,ELISA,IP,IF-Cell

Conjugate :

Non-conjugated

Immunogen :

Synthetic peptide EQKLISEEDL conjugated to keyhole limpet haemocyanin.

Uniprot id :

Host :

Mouse

Clone number :

A3C8

Isotype :

IgG1

Size :

100μl

List Price :

360.00 USD

Storage Buffer :

1*TBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.

Form :

Liquid

Storage Instruction :

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.

Purity :

Protein A affinity purified.

Product type :

Mouse monoclonal Antibody

Positive control :

C-terminal Myc-tagged recombinant protein lysates, N-terminal Myc-tagged recombinant protein lysates.

Concentration :

2 mg/mL.

Recommended dilutions :

WB: 1:50,000-1:100,000 ;IF-Cell: 1:1,000 ;IP: 2-5 µg/ml. ;ELISA: Use at an assay dependent concentration.

Pic img4 :

https://storage.huabio.cn/huabio/productImg/EM1902-38_4.jpg

Pic legend4 :

Myc tag was immunoprecipitated in 2µg C terminal Myc Tag fusion protein lysate with EM1902-38 at 2 µg/20 µl agarose. Western blot was performed from the immunoprecipitate using R1208-1 at 1/1,000 dilution. Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1:100,000 dilution was used for 60 mins at room temperature. Lane 1: Myc Tag fusion protein lysate (input). Lane 2: EM1902-38 IP in Myc Tag fusion protein lysate. Lane 3: EM31105 IP in Myc Tag fusion protein lysate. Lane 4: Mouse IgG instead of EM1902-38 in Myc Tag fusion protein lysate. Blocking/Dilution buffer: 5% NFDM/TBST

Pic img5 :

https://storage.huabio.cn/huabio/productImg/EM1902-38_5.jpg

Pic legend5 :

Myc tag was immunoprecipitated in 2µg N terminal Myc Tag fusion protein lysate with EM1902-38 at 2 µg/20 µl agarose. Western blot was performed from the immunoprecipitate using R1208-1 at 1/1,000 dilution. Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1:100,000 dilution was used for 60 mins at room temperature. Lane 1: Myc Tag fusion protein lysate (input). Lane 2: EM1902-38 IP in Myc Tag fusion protein lysate. Lane 3: EM31105 IP in Myc Tag fusion protein lysate. Lane 4: Mouse IgG instead of EM1902-38 in Myc Tag fusion protein lysate. Blocking/Dilution buffer: 5% NFDM/TBST

more info or order :

HUABIO product webpage