CD11b | HUABIO EM1902-35 product information

product summary

company name :

HUABIO

product type :

antibody

product name :

CD11b

catalog :

EM1902-35

quantity :

100μl

price :

360.00 USD

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

A2-B5-D2

reactivity :

human, mouse

application :

western blot

more info or order :

HUABIO product webpage

image

image 1 :

Western blot analysis of CD11b on different lysates with Mouse anti-CD11b antibody (EM1902-35) at 1/500 dilution. Lane 1: RAW264.7 cell lysate (RIPA lysis) Lane 2: RAW264.7 cell lysate (hot lysis) Lane 3: RAW264.7 cell lysate (no heat) Notice: no heat means the lysate is not boiled. Lysates/proteins at 10 µg/Lane. Predicted band size: 127 kDa Observed band size: 170 kDa Exposure time: 3 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (EM1902-35) at 1/500 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1/100,000 dilution was used for 1 hour at room temperature.

image 2 :

Western blot analysis of CD11b on Mouse spleen tissue lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (EM1902-35, 1/100) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1:5,000 dilution was used for 1 hour at room temperature.

product information

SKU :

EM1902-35

Target name :

CD11b

Species reactivity :

Human,Mouse

Applications :

Conjugate :

Non-conjugated

Immunogen :

Recombinant protein within mouse CD11b aa 1-300.

Uniprot id :

P11215>SwissProt: P11215 Human;SwissProt: P05555 Mouse

Host :

Mouse

Clone number :

A2-B5-D2

Isotype :

IgM

Size :

100μl

List Price :

360.00 USD

Storage Buffer :

1*TBS (pH7.4), 1%BSA, 50%Glycerol. Preservative: 0.05% Sodium Azide.

Form :

Liquid

Storage Instruction :

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.

Purity :

Protein A/G affinity purified.

Product type :

Mouse monoclonal Antibody

Positive control :

RAW264.7 cell lysates, Mouse spleen tissue lysates, A549.

Molecular wt :

Predicted band size: 127 kDa.

Subcellular location :

Cell membrane, Membrane.

Concentration :

2 mg/mL.

Recommended dilutions :

WB: 1:500

more info or order :

HUABIO product webpage