ApoER2 | HUABIO EM1902-30 product information

product summary

company name :

HUABIO

product type :

antibody

product name :

ApoER2

catalog :

EM1902-30

quantity :

100μl

price :

360.00 USD

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

13B2

reactivity :

human

application :

western blot, immunohistochemistry - paraffin section

more info or order :

HUABIO product webpage

image

image 1 :

Western blot analysis of ApoER2 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (EM1902-30, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1:5,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: A549 cell lysate Lane 2: SHSY5Y cell lysate Lane 3: SKBR-3 cell lysate Lane 4: U937 cell lysate

image 2 :

Immunohistochemical analysis of paraffin-embedded human testis tissue with Mouse anti-ApoER2 antibody (EM1902-30) at 1/400 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (EM1902-30) at 1/400 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

product information

SKU :

EM1902-30

Target name :

ApoER2

Species reactivity :

Human

Applications :

WB,IHC-P

Conjugate :

Non-conjugated

Immunogen :

Recombinant protein within Human ApoER2 aa 47-275 / 963.

Uniprot id :

Q14114>SwissProt: Q14114 Human

Host :

Mouse

Clone number :

13B2

Isotype :

IgG2a

Size :

100μl

List Price :

360.00 USD

Storage Buffer :

1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.

Form :

Liquid

Storage Instruction :

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.

Purity :

Protein A affinity purified.

Product type :

Mouse monoclonal Antibody

Positive control :

A549 cell lysate, SHSY5Y cell lysate, SKBR-3 cell lysate, U937 cell lysate, Hela, A549, human testis tissue.

Molecular wt :

Predicted band size: 106 kDa

Subcellular location :

Cell membrane, Secreted.

Concentration :

2 mg/ml.

Recommended dilutions :

WB: 1:500 ;IHC-P: 1:400

more info or order :

HUABIO product webpage