MSH6 | HUABIO EM1902-24 product information

product summary

company name :

HUABIO

product type :

antibody

product name :

MSH6

catalog :

EM1902-24

quantity :

100μl

price :

360.00 USD

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

A4C4

reactivity :

human, mouse

application :

western blot, immunoprecipitation, immunohistochemistry - paraffin section

more info or order :

HUABIO product webpage

image

image 1 :

Western blot analysis of MSH6 on different lysates with Mouse anti-MSH6 antibody (EM1902-24) at 1/1,000 dilution. Lane 1: SH-SY5Y cell lysate Lane 2: 293T cell lysate Lane 3: NIH/3T3 cell lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 153 kDa Observed band size: 180/140 kDa Exposure time: 15 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (EM1902-24) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1/100,000 dilution was used for 1 hour at room temperature.

image 2 :

Immunocytochemistry analysis of A549 cells labeling MSH6 with Mouse anti-MSH6 antibody (EM1902-24) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Mouse anti-MSH6 antibody (EM1902-24) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Mouse IgG H&L (iFluor™ 488, HA1125) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. beta Tubulin (ET1602-4, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Rabbit IgG H&L (iFluor™ 594, HA1122) was used as the secondary antibody at 1/1,000 dilution.

image 3 :

Immunocytochemistry analysis of RAW264.7 cells labeling MSH6 with Mouse anti-MSH6 antibody (EM1902-24) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Mouse anti-MSH6 antibody (EM1902-24) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Mouse IgG H&L (iFluor™ 488, HA1125) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. beta Tubulin (ET1602-4, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Rabbit IgG H&L (iFluor™ 594, HA1122) was used as the secondary antibody at 1/1,000 dilution.

product information

SKU :

EM1902-24

Target name :

MSH6

Species reactivity :

Human,Mouse

Applications :

WB,IHC-P,IF-Tissue,IF-Cell,IP

Conjugate :

Non-conjugated

Immunogen :

Synthetic peptide corresponding to C-terminal Human MSH6.

Uniprot id :

P52701>SwissProt: P52701 Human;SwissProt: P54276 Mouse

Host :

Mouse

Clone number :

A4C4

Isotype :

IgG2b

Size :

100μl

List Price :

360.00 USD

Storage Buffer :

1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.

Form :

Liquid

Storage Instruction :

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.

Purity :

Protein G affinity purified.

Product type :

Mouse monoclonal Antibody

Positive control :

SH-SY5Y cell lysate, 293T cell lysate, NIH/3T3 cell lysate, A549, RAW264.7, human breast carcinoma tissue, mouse testis tissue, human thyroid tissue, human colon carcinoma tissue, human skin tissue, human esophagus tissue, human placenta tissue, mouse colon tissue, mouse placenta tissue, human gastric carcinoma tissue, mouse spleen tissue, mouse small intestine tissue.

Molecular wt :

Predicted band size: 153 kDa

Subcellular location :

Nucleus, Chromosome.

Concentration :

2 mg/mL.

Recommended dilutions :

WB: 1:500-1:2,000 ;IF-Tissue: 1:50 ;IHC-P: 1:200-1:1,000 ;IF-Cell: 1:100 ;IP: 1-2μg/sample

Pic img4 :

https://storage.huabio.cn/huabio/productImg/EM1902-24_4.jpg

Pic legend4 :

Immunofluorescence analysis of paraffin-embedded human breast carcinoma tissue labeling MSH6 with Mouse anti-MSH6 antibody (EM1902-24) at 1/50 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS, and then probed with the primary antibody (EM1902-24, green) at 1/50 dilution overnight at 4 ℃, washed with PBS. Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) was used as the secondary antibody at 1/1,000 dilution. Nuclei were counterstained with DAPI (blue).

Pic img5 :

https://storage.huabio.cn/huabio/productImg/EM1902-24_5.jpg

Pic legend5 :

Immunofluorescence analysis of paraffin-embedded mouse testis tissue labeling MSH6 with Mouse anti-MSH6 antibody (EM1902-24) at 1/50 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS, and then probed with the primary antibody (EM1902-24, green) at 1/50 dilution overnight at 4 ℃, washed with PBS. Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) was used as the secondary antibody at 1/1,000 dilution. Nuclei were counterstained with DAPI (blue).

Pic img6 :

https://storage.huabio.cn/huabio/productImg/EM1902-24_6.jpg

Pic legend6 :

Immunohistochemical analysis of paraffin-embedded human thyroid tissue with Mouse anti-MSH6 antibody (EM1902-24) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (EM1902-24) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Pic img7 :

https://storage.huabio.cn/huabio/productImg/EM1902-24_7.jpg

Pic legend7 :

Immunohistochemical analysis of paraffin-embedded human colon carcinoma tissue with Mouse anti-MSH6 antibody (EM1902-24) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (EM1902-24) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Pic img8 :

https://storage.huabio.cn/huabio/productImg/EM1902-24_8.jpg

Pic legend8 :

Immunohistochemical analysis of paraffin-embedded human skin tissue with Mouse anti-MSH6 antibody (EM1902-24) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (EM1902-24) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Pic img9 :

https://storage.huabio.cn/huabio/productImg/EM1902-24_9.jpg

Pic legend9 :

Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue with Mouse anti-MSH6 antibody (EM1902-24) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (EM1902-24) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Pic img10 :

https://storage.huabio.cn/huabio/productImg/EM1902-24_10.jpg

Pic legend10 :

Immunohistochemical analysis of paraffin-embedded human esophagus tissue with Mouse anti-MSH6 antibody (EM1902-24) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (EM1902-24) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Pic img11 :

https://storage.huabio.cn/huabio/productImg/EM1902-24_11.jpg

Pic legend11 :

Immunohistochemical analysis of paraffin-embedded human placenta tissue with Mouse anti-MSH6 antibody (EM1902-24) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (EM1902-24) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Pic img12 :

https://storage.huabio.cn/huabio/productImg/EM1902-24_12.jpg

Pic legend12 :

Immunohistochemical analysis of paraffin-embedded mouse testis tissue with Mouse anti-MSH6 antibody (EM1902-24) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (EM1902-24) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Pic img13 :

https://storage.huabio.cn/huabio/productImg/EM1902-24_13.jpg

Pic legend13 :

Immunohistochemical analysis of paraffin-embedded mouse colon tissue with Mouse anti-MSH6 antibody (EM1902-24) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (EM1902-24) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Pic img14 :

https://storage.huabio.cn/huabio/productImg/EM1902-24_14.jpg

Pic legend14 :

Immunohistochemical analysis of paraffin-embedded mouse placenta tissue with Mouse anti-MSH6 antibody (EM1902-24) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (EM1902-24) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Pic img15 :

https://storage.huabio.cn/huabio/productImg/EM1902-24_15.jpg

Pic legend15 :

Immunohistochemical analysis of paraffin-embedded human gastric carcinoma tissue with Mouse anti-MSH6 antibody (EM1902-24) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (EM1902-24) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Pic img16 :

https://storage.huabio.cn/huabio/productImg/EM1902-24_16.jpg

Pic legend16 :

Immunohistochemical analysis of paraffin-embedded mouse spleen tissue with Mouse anti-MSH6 antibody (EM1902-24) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (EM1902-24) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Pic img17 :

https://storage.huabio.cn/huabio/productImg/EM1902-24_17.jpg

Pic legend17 :

Immunohistochemical analysis of paraffin-embedded mouse small intestine tissue with Mouse anti-MSH6 antibody (EM1902-24) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (EM1902-24) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

more info or order :

HUABIO product webpage