YKL-40 / CHI3L1 | HUABIO EM1902-14-50UL product information

product summary

company name :

HUABIO

product type :

antibody

product name :

YKL-40 / CHI3L1

catalog :

EM1902-14-50UL

quantity :

50μl

price :

205.00 USD

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

10C1

reactivity :

human, mouse

application :

western blot, flow cytometry, immunohistochemistry - paraffin section

more info or order :

HUABIO product webpage

image

image 1 :

Western blot analysis of YKL-40 / CHI3L1 on THP-1 cell lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (EM1902-14, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1:5,000 dilution was used for 1 hour at room temperature.

image 2 :

Application: IF-Tissue Species: Mouse Site: lung Sample: Paraffin-embedded section Antibody concentration: 1/200

image 3 :

Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-YKL-40 / CHI3L1 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the antibody (EM1902-14) at 1/50 dilution, for 30 minutes at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chrogen. Counter stained with hematoxylin and mounted with DPX.

product information

SKU :

EM1902-14-50UL

Target name :

YKL-40 / CHI3L1

Species reactivity :

Human,Mouse

Applications :

WB,IHC-P,IF-Cell,FC,IF-Tissue

Conjugate :

Non-conjugated

Immunogen :

Synthetic peptide within Human YKL-40 aa 24-73 / 383.

Uniprot id :

P36222>SwissProt: P36222 Human;SwissProt: Q61362 Mouse

Host :

Mouse

Clone number :

10C1

Isotype :

IgG1

Size :

50μl

List Price :

205.00 USD

Storage Buffer :

1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.

Form :

Liquid

Storage Instruction :

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.

Purity :

Protein G affinity purified.

Product type :

Mouse monoclonal Antibody

Positive control :

THP-1 cell lysates, human tonsil tissue, human liver tissue, mouse lung tissue, HepG2.

Molecular wt :

Predicted band size: 43 kDa

Subcellular location :

Cytoplasm, Endoplasmic reticulum, Secreted.

Concentration :

2 mg/mL.

Recommended dilutions :

WB: 1:500-1:1,000 ;IHC-P: 1:50-1:200 ;IF-Cell: 1:100 ;FC: 1:50-1:100 ;IF-Tissue: 1:200

Pic img4 :

https://storage.huabio.cn/huabio/productImg/EM1902-14_4.jpg

Pic legend4 :

Immunohistochemical analysis of paraffin-embedded human liver tissue using anti-YKL-40 / CHI3L1 antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the antibody (EM1902-14) at 1/200 dilution, for 30 minutes at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chrogen. Counter stained with hematoxylin and mounted with DPX.

Pic img5 :

https://storage.huabio.cn/huabio/productImg/EM1902-14_5.jpg

Pic legend5 :

Immunohistochemical analysis of paraffin-embedded mouse lung tissue using anti-YKL-40 / CHI3L1 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the antibody (EM1902-14) at 1/200 dilution, for 30 minutes at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chrogen. Counter stained with hematoxylin and mounted with DPX.

Pic img6 :

https://storage.huabio.cn/huabio/productImg/EM1902-14_6.jpg

Pic legend6 :

Flow cytometric analysis of YKL-40 / CHI3L1 was done on HepG2 cells. The cells were fixed, permeabilized and stained with YKL-40 / CHI3L1 antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). After incubation of the primary antibody on room temperature for an hour, the cells was stained with a Alexa Fluor™ 488-conjugated goat anti-mouse IgG Secondary antibody at 1/500 dilution for 30 minutes.

Pic img7 :

https://storage.huabio.cn/huabio/productImg/EM1902-14_7.jpg

Pic legend7 :

Immunocytochemistry analysis of THP-1 cells labeling YKL-40 / CHI3L1 with Mouse anti-YKL-40 / CHI3L1 antibody (EM1902-14) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Mouse anti-YKL-40 / CHI3L1 antibody (EM1902-14) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Mouse IgG H&L (iFluor™ 488, HA1125) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. beta Tubulin (ET1602-4, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Rabbit IgG H&L (iFluor™ 594, HA1122) were used as the secondary antibody at 1/1,000 dilution.

more info or order :

HUABIO product webpage