Caspr | HUABIO EM1902-10 product information

product summary

company name :

HUABIO

product type :

antibody

product name :

Caspr

catalog :

EM1902-10

quantity :

100μl

price :

360.00 USD

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

A2D2

reactivity :

human, mouse

application :

flow cytometry, immunohistochemistry - paraffin section

more info or order :

HUABIO product webpage

image

image 1 :

Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-Caspr antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (EM1902-10, 1/200) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

image 2 :

Immunohistochemical analysis of paraffin-embedded mouse kidney tissue using anti-Caspr antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (EM1902-10, 1/200) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

image 3 :

Flow cytometric analysis of Caspr was done on SH-SY5Y cells. The cells were fixed, permeabilized and stained with the primary antibody (EM1902-10, 1/50) (red). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated Goat anti-Mouse IgG Secondary antibody at 1/1000 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; black).

product information

SKU :

EM1902-10

Target name :

Caspr

Species reactivity :

Human,Mouse

Applications :

IHC-P,FC

Conjugate :

Non-conjugated

Immunogen :

Recombinant protein within human Caspr aa 1100-1300.

Uniprot id :

P78357>SwissProt: P78357 Human;SwissProt: O54991 Mouse

Host :

Mouse

Clone number :

A2D2

Isotype :

IgG1

Size :

100μl

List Price :

360.00 USD

Storage Buffer :

1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.

Form :

Liquid

Storage Instruction :

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.

Purity :

Protein G affinity purified.

Product type :

Mouse monoclonal Antibody

Positive control :

Human kidney tissue, mouse kidney tissue, SH-SY5Y.

Molecular wt :

Predicted band size: 156 kDa.

Subcellular location :

Membrane, paranodal septate junction.

Concentration :

2 mg/mL.

Recommended dilutions :

IHC-P:1:50-1:200 ;FC:1:50-1:100

more info or order :

HUABIO product webpage