HMGB2 | HUABIO EM1902-06 product information

product summary

company name :

HUABIO

product type :

antibody

product name :

HMGB2

catalog :

EM1902-06

quantity :

100μl

price :

360.00 USD

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

10D1

reactivity :

human, mouse, rat

application :

western blot, immunohistochemistry - paraffin section

more info or order :

HUABIO product webpage

image

image 1 :

Western blot analysis of HMGB2 on different lysates with Mouse anti-HMGB2 antibody (EM1902-06) at 1/1,000 dilution. Lane 1: HeLa cell lysate Lane 2: K-562 cell lysate Lane 3: 293T cell lysate Lane 4: RAW264.7 cell lysate Lane 5: PC-12 cell lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 24 kDa Observed band size: 24 kDa Exposure time: 3 minutes; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (EM1902-06) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1/50,000 dilution was used for 1 hour at room temperature.

image 2 :

Western blot analysis of HMGB2 on different lysates with Mouse anti-HMGB2 antibody (EM1902-06) at 1/2,000 dilution. Lane 1: HAP1-parental cell lysate Lane 2: HAP1-HMGB2 KD cell lysate Lysates/proteins at 10 µg/Lane. Predicted band size: 24 kDa Observed band size: 24 kDa Exposure time: 9 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (EM1902-06) at 1/2,000 dilution was used in K1803 at 4℃ overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1/50,000 dilution was used for 1 hour at room temperature.

image 3 :

Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-HMGB2 antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (EM1902-06, 1/1,000) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

product information

SKU :

EM1902-06

Target name :

HMGB2

Species reactivity :

Human,Mouse,Rat

Applications :

WB,IHC-P

Conjugate :

Non-conjugated

Immunogen :

Recombinant protein within Human HMGB2 aa 1-209 / 209.

Uniprot id :

P26583>SwissProt: P26583 Human;SwissProt: P30681 Mouse;SwissProt: P52925 Rat

Host :

Mouse

Clone number :

10D1

Isotype :

IgG2b

Size :

100μl

List Price :

360.00 USD

Storage Buffer :

1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.

Form :

Liquid

Storage Instruction :

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.

Purity :

Protein G affinity purified.

Product type :

Mouse monoclonal Antibody

Positive control :

HeLa cell lysate, K-562 cell lysate, 293T cell lysate, RAW264.7 cell lysate, PC-12 cell lysate, human tonsil tissue, human cervical tissue, human thyroid tissue, human colon carcinoma tissue, human spleen tissue, human prostate carcinoma tissue, human breast carcinoma tissue, mouse liver tissue, mouse testis tissue, mouse fallopian tube tissue, mouse small intestine tissue.

Molecular wt :

Predicted band size: 24 kDa

Subcellular location :

Nucleus, secreted, chromosome, cytoplasm.

Concentration :

2 mg/mL.

Recommended dilutions :

WB: 1:1,000-1:2,000 ;IHC-P: 1:200-1:1,000

Advanced Validation :

Knockdown (KD)

Pic img4 :

https://storage.huabio.cn/huabio/productImg/EM1902-06_4.jpg

Pic legend4 :

Immunohistochemical analysis of paraffin-embedded human cervical tissue using anti-HMGB2 antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (EM1902-06, 1/1,000) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Pic img5 :

https://storage.huabio.cn/huabio/productImg/EM1902-06_5.jpg

Pic legend5 :

Immunohistochemical analysis of paraffin-embedded human thyroid tissue using anti-HMGB2 antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (EM1902-06, 1/1,000) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Pic img6 :

https://storage.huabio.cn/huabio/productImg/EM1902-06_6.jpg

Pic legend6 :

Immunohistochemical analysis of paraffin-embedded human colon carcinoma tissue using anti-HMGB2 antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (EM1902-06, 1/1,000) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Pic img7 :

https://storage.huabio.cn/huabio/productImg/EM1902-06_7.jpg

Pic legend7 :

Immunohistochemical analysis of paraffin-embedded human spleen tissue using anti-HMGB2 antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (EM1902-06, 1/1,000) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Pic img8 :

https://storage.huabio.cn/huabio/productImg/EM1902-06_8.jpg

Pic legend8 :

Immunohistochemical analysis of paraffin-embedded human prostate carcinoma tissue using anti-HMGB2 antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (EM1902-06, 1/1,000) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Pic img9 :

https://storage.huabio.cn/huabio/productImg/EM1902-06_9.jpg

Pic legend9 :

Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using anti-HMGB2 antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (EM1902-06, 1/1,000) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Pic img10 :

https://storage.huabio.cn/huabio/productImg/EM1902-06_10.jpg

Pic legend10 :

Immunohistochemical analysis of paraffin-embedded mouse liver tissue using anti-HMGB2 antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (EM1902-06, 1/1,000) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Pic img11 :

https://storage.huabio.cn/huabio/productImg/EM1902-06_11.jpg

Pic legend11 :

Immunohistochemical analysis of paraffin-embedded mouse testis tissue using anti-HMGB2 antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (EM1902-06, 1/1,000) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Pic img12 :

https://storage.huabio.cn/huabio/productImg/EM1902-06_12.jpg

Pic legend12 :

Immunohistochemical analysis of paraffin-embedded mouse fallopian tube tissue using anti-HMGB2 antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (EM1902-06, 1/1,000) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Pic img13 :

https://storage.huabio.cn/huabio/productImg/EM1902-06_13.jpg

Pic legend13 :

Immunohistochemical analysis of paraffin-embedded mouse small intestine tissue using anti-HMGB2 antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (EM1902-06, 1/1,000) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

more info or order :

HUABIO product webpage