Lysozyme | HUABIO EM1901-98 product information

product summary

company name :

HUABIO

product type :

antibody

product name :

Lysozyme

catalog :

EM1901-98

quantity :

100μl

price :

360.00 USD

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

A5A8

reactivity :

human

application :

western blot, immunohistochemistry - paraffin section

more info or order :

HUABIO product webpage

image

image 1 :

Western blot analysis of Lysozyme on different lysates with Mouse anti-Lysozyme antibody (EM1901-98) at 1/5,000 dilution. Lane 1: HepG2 cell lysate Lane 2: HL-60 cell lysate Lysates/proteins at 15 µg/Lane. Predicted band size: 17 kDa Observed band size: 14 kDa Exposure time: 3 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (EM1901-98) at 1/5,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1/50,000 dilution was used for 1 hour at room temperature.

image 2 :

Immunohistochemical analysis of paraffin-embedded human spleen tissue with Mouse anti-Lysozyme antibody (EM1901-98) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (EM1901-98) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

image 3 :

Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue with Mouse anti-Lysozyme antibody (EM1901-98) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (EM1901-98) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

product information

SKU :

EM1901-98

Target name :

Lysozyme

Species reactivity :

Human

Applications :

WB,IHC-P

Conjugate :

Non-conjugated

Immunogen :

Recombinant protein within Human Lysozyme aa 1-141 / 148.

Uniprot id :

P61626>SwissProt: P61626 Human

Host :

Mouse

Clone number :

A5A8

Isotype :

IgG1

Size :

100μl

List Price :

360.00 USD

Storage Buffer :

1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.

Form :

Liquid

Storage Instruction :

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.

Purity :

Protein G affinity purified.

Product type :

Mouse monoclonal Antibody

Positive control :

HepG2 cell lysate, HL-60 cell lysate, human spleen tissue, human stomach cancer tissue.

Molecular wt :

Predicted band size: 17 kDa

Subcellular location :

Secreted.

Concentration :

2 mg/mL.

Recommended dilutions :

WB: 1:5,000 ;IHC-P: 1:1,000

more info or order :

HUABIO product webpage