CD68 | HUABIO EM1901-95 product information

product summary

company name :

HUABIO

product type :

antibody

product name :

CD68

catalog :

EM1901-95

quantity :

100μl

price :

360.00 USD

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

A3C4

reactivity :

human

application :

western blot, flow cytometry, immunohistochemistry - paraffin section

more info or order :

HUABIO product webpage

image

image 1 :

Western blot analysis of CD68 on different lysates with Mouse anti-CD68 antibody (EM1901-95) at 1/1,000 dilution. Lane 1: A431 cell lysate Lane 2: THP-1 cell lysate Lane 3: U-937 cell lysate Lane 4: Jurkat cell lysate (negative) Lysates/proteins at 20 µg/Lane. Predicted band size: 37 kDa Observed band size: 100-150 kDa Exposure time: 3 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (EM1901-95) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1/50,000 dilution was used for 1 hour at room temperature.

image 2 :

Fluorescence multiplex immunohistochemical analysis of the human pancreatic carcinoma (Formalin/PFA-fixed paraffin-embedded sections). Panel A: the merged image of anti-CD68 (EM1901-95, green), anti-CD163 (ET1704-43, red) and anti-PanCK (HA601094, violet) on human pancreatic carcinoma. Panel B: anti- CD68 stained on M1 macrophages. Panel C: anti-CD163 stained on M2 macrophages cells. Panel D: anti-panCK stained on cancer cells. HRP Conjugated UltraPolymer Goat Polyclonal Antibody HA1119/HA1120 was used as a secondary antibody. The immunostaining was performed with the Sequential Immuno-staining Kit (IRISKitCmTSA Kit 900803). The section was incubated in three rounds of staining: in the order of EM1901-95 (1/3,000 dilution), ET1704-43 (1/3,000 dilution), and HA601094 (1/3,000 dilution) for 20 mins at room temperature. Each round was followed by a separate fluorescent tyramide signal amplification system. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 30 mins at 95℃. DAPI (blue) was used as a nuclear counter stain. Image acquisition was performed with Nikon ECLIPSE Ni-E microscope.

image 3 :

Fluorescence multiplex immunohistochemical analysis of human colon carcinoma (Formalin/PFA-fixed paraffin-embedded sections). Panel A: the merged image of anti-CD68 (EM1901-95, Yellow) and anti-CD56 (ET1702-43, Red) on human colon carcinoma. HRP Conjugated UltraPolymer Goat Polyclonal Antibody HA1119/HA1120 was used as a secondary antibody. The immunostaining was performed with the Sequential Immuno-staining Kit (IRISKit™MH010101, www.luminiris.cn). The section was incubated in two rounds of staining: in the order of EM1901-95 (1/3,000 dilution) and ET1702-43 (1/1,000 dilution) for 20 mins at room temperature. Each round was followed by a separate fluorescent tyramide signal amplification system. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 30 mins at 95℃. DAPI (blue) was used as a nuclear counter stain. Image acquisition was performed with Zeiss Observer 7 Inverted Fluorescence Microscope.

product information

SKU :

EM1901-95

Target name :

CD68

Species reactivity :

Human

Applications :

WB,IHC-P,FC,mIHC

Conjugate :

Non-conjugated

Immunogen :

Synthetic peptide within human CD68 aa 320-354.

Uniprot id :

P34810>SwissProt: P34810 Human

Host :

Mouse

Clone number :

A3C4

Isotype :

IgG1

Size :

100μl

List Price :

360.00 USD

Storage Buffer :

1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.

Form :

Liquid

Storage Instruction :

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.

Purity :

Protein G affinity purified.

Product type :

Mouse monoclonal Antibody

Positive control :

A431 cell lysate, THP-1 cell lysate, U-937 cell lysate, human tonsil tissue, human lung tissue, THP-1, human pancreatic carcinoma.

Molecular wt :

Predicted band size: 37 kDa.

Subcellular location :

Cell membrane. Endosome membrane, lysosome membrane.

Concentration :

2 mg/mL.

Recommended dilutions :

WB:1:1,000-1:2,000 ;IHC-P:1:200-1:1,000 ;FC:1:1,000 ;mIHC: 1:3,000

Advanced Validation :

Relative expression (RE)

Pic img4 :

https://storage.huabio.cn/huabio/productImg/EM1901-95_4.jpg

Pic legend4 :

Fluorescence multiplex immunohistochemical analysis of human cervical carcinoma (Formalin/PFA-fixed paraffin-embedded sections). Panel A: the merged image of anti-CD66b (HA500100, Green), anti-CD11b (ET1706-04, Red) and anti-CD68 (EM1901-95, Yellow) on human cervical carcinoma. HRP Conjugated UltraPolymer Goat Polyclonal Antibody HA1119/HA1120 was used as a secondary antibody. The immunostaining was performed with the Sequential Immuno-staining Kit (IRISKit™MH010101, www.luminiris.cn). The section was incubated in three rounds of staining: in the order of HA500100 (1/1,000 dilution), ET1706-04 (1/1,000 dilution) and EM1901-95 (1/3,000 dilution) for 20 mins at room temperature. Each round was followed by a separate fluorescent tyramide signal amplification system. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 30 mins at 95℃. DAPI (blue) was used as a nuclear counter stain. Image acquisition was performed with Zeiss Observer 7 Inverted Fluorescence Microscope.

Pic img5 :

https://storage.huabio.cn/huabio/productImg/EM1901-95_5.jpg

Pic legend5 :

Immunohistochemical analysis of paraffin-embedded human tonsil tissue with Mouse anti-CD68 antibody (EM1901-95) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (EM1901-95) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Pic img6 :

https://storage.huabio.cn/huabio/productImg/EM1901-95_6.jpg

Pic legend6 :

Immunohistochemical analysis of paraffin-embedded human lung tissue using anti-CD68 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (EM1901-95, 1/200) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Pic img7 :

https://storage.huabio.cn/huabio/productImg/EM1901-95_7.jpg

Pic legend7 :

Flow cytometric analysis of THP-1 cells labeling CD68. Cells were fixed and permeabilized. Then stained with the primary antibody (EM1901-95, 1/1,000) (red) compared with Mouse IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Mouse IgG Secondary antibody (HA1125) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).

more info or order :

HUABIO product webpage