ACY1 | HUABIO EM1901-86 product information

product summary

company name :

HUABIO

product type :

antibody

product name :

ACY1

catalog :

EM1901-86

quantity :

100μl

price :

360.00 USD

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

15G2

reactivity :

human, mouse, rat

application :

western blot, flow cytometry, immunohistochemistry - paraffin section

more info or order :

HUABIO product webpage

image

image 1 :

Western blot analysis of ACY1 on different lysates with Mouse anti-ACY1 antibody (EM1901-86) at 1/1,000 dilution. Lane 1: K-562 cell lysate (20 µg/Lane) Lane 2: HepG2 cell lysate (20 µg/Lane) Lane 3: PC-3M cell lysate (20 µg/Lane) Lane 4: Human liver tissue lysate (40 µg/Lane) Lane 5: Human kidney tissue lysate (40 µg/Lane) Lane 6: Mouse liver tissue lysate (40 µg/Lane) Lane 7: Mouse kidney tissue lysate (40 µg/Lane) Lane 8: Rat liver tissue lysate (40 µg/Lane) Lane 9: Rat kidney tissue lysate (40 µg/Lane) Predicted band size: 46 kDa Observed band size: 42 kDa Exposure time: 3 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (EM1901-86) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1/50,000 dilution was used for 1 hour at room temperature.

image 2 :

Western blot analysis of ACY1 on different lysates with Mouse anti-ACY1 antibody (EM1901-86) at 1/1,000 dilution. Lane 1: A549-si NT cell lysate Lane 2: A549-si ACY1 cell lysate Lysates/proteins at 10 µg/Lane. Predicted band size: 46 kDa Observed band size: 46 kDa Exposure time: 3 minutes; ECL: K1802; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (EM1901-86) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1/50,000 dilution was used for 1 hour at room temperature.

image 3 :

Immunohistochemical analysis of paraffin-embedded human kidney tissue with Mouse anti-ACY1 antibody (EM1901-86) at 1/2,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (EM1901-86) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

product information

SKU :

EM1901-86

Target name :

ACY1

Species reactivity :

Human,Mouse,Rat

Applications :

WB,IHC-P,FC

Conjugate :

Non-conjugated

Immunogen :

Recombinant protein within Human ACY1 aa 55-256 / 408.

Uniprot id :

Q03154>SwissProt: Q03154 Human;SwissProt: Q99JW2 Mouse;SwissProt: Q6AYS7 Rat

Host :

Mouse

Clone number :

15G2

Isotype :

IgG2a

Size :

100μl

List Price :

360.00 USD

Storage Buffer :

1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.

Form :

Liquid

Storage Instruction :

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.

Purity :

Protein A affinity purified.

Product type :

Mouse monoclonal Antibody

Positive control :

K-562 cell lysate, HepG2 cell lysate, PC-3M cell lysate, human liver tissue lysate, human kidney tissue lysate, mouse liver tissue lysate, mouse kidney tissue lysate, rat liver tissue lysate, rat kidney tissue lysate, human kidney tissue, mouse kidney tissue, rat kidney tissue, SHSY5Y.

Molecular wt :

Predicted band size: 46 kDa

Subcellular location :

Cytoplasm

Concentration :

2 mg/ml.

Recommended dilutions :

WB: 1:500-1:1,000 ;IHC-P: 1:2,000 ;FC: 1:50-1:100

Advanced Validation :

Knockdown (KD)

Pic img4 :

https://storage.huabio.cn/huabio/productImg/EM1901-86_4.jpg

Pic legend4 :

Immunohistochemical analysis of paraffin-embedded mouse kidney tissue with Mouse anti-ACY1 antibody (EM1901-86) at 1/2,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (EM1901-86) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Pic img5 :

https://storage.huabio.cn/huabio/productImg/EM1901-86_5.jpg

Pic legend5 :

Immunohistochemical analysis of paraffin-embedded rat kidney tissue with Mouse anti-ACY1 antibody (EM1901-86) at 1/2,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (EM1901-86) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Pic img6 :

https://storage.huabio.cn/huabio/productImg/EM1901-86_6.jpg

Pic legend6 :

Flow cytometric analysis of ACY1 was done on SHSY5Y cells. The cells were fixed, permeabilized and stained with the primary antibody (EM1901-86, 1/100) (red). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa FluorTM488 Goat anti-Mouse IgG IgG Secondary antibody at 1/500 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; black).

more info or order :

HUABIO product webpage