Albumin | HUABIO EM1901-80 product information

product summary

company name :

HUABIO

product type :

antibody

product name :

Albumin

catalog :

EM1901-80

quantity :

100μl

price :

360.00 USD

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

C5-A8

reactivity :

human, rat

application :

western blot, ELISA, flow cytometry, immunohistochemistry - paraffin section

more info or order :

HUABIO product webpage

image

image 1 :

Western blot analysis of Albumin on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (EM1901-80, 1/50000) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1:5,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: Human plasma sample Lane 2: HepG2 cell lysate

image 2 :

Immunohistochemical analysis of paraffin-embedded rat kidney tissue using anti-Albumin antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (EM1901-80, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

image 3 :

Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue using anti-Albumin antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (EM1901-80, 1/100) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

product information

SKU :

EM1901-80

Target name :

Albumin

Species reactivity :

Human,Rat

Applications :

WB,IHC-P,FC,ELISA

Conjugate :

Non-conjugated

Immunogen :

Native protein.

Uniprot id :

P02768>SwissProt: P02768 Human;SwissProt: P02770 Rat

Host :

Mouse

Clone number :

C5-A8

Isotype :

IgG1

Size :

100μl

List Price :

360.00 USD

Storage Buffer :

1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.

Form :

Liquid

Storage Instruction :

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.

Purity :

Protein A affinity purified.

Product type :

Mouse monoclonal Antibody

Positive control :

Human plasma tissue lysate, HepG2 cell lysate, rat kidney tissue, human prostate cancer tissue, human placenta tissue, HepG2.

Molecular wt :

Predicted band size: 69 kDa

Subcellular location :

Secreted.

Concentration :

2mg/ml.

Recommended dilutions :

WB: 1:10,000-1:100,000 ;IHC-P: 1:50-1:100 ;FC: 1:50-1:100 ;ELISA: Use at an assay dependent concentration.

Pic img4 :

https://storage.huabio.cn/huabio/productImg/EM1901-80_4.jpg

Pic legend4 :

Immunohistochemical analysis of paraffin-embedded human placenta tissue using anti-Albumin antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (EM1901-80, 1/100) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Pic img5 :

https://storage.huabio.cn/huabio/productImg/EM1901-80_5.jpg

Pic legend5 :

Flow cytometric analysis of Albumin was done on HepG2 cells. The cells were fixed, permeabilized and stained with the primary antibody (EM1901-80, 1/100) (red). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa FluorTM488 Goat anti-Mouse IgG Secondary antibody at 1/500 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; black).

more info or order :

HUABIO product webpage