Integrin beta 1 | HUABIO EM1901-77 product information

product summary

company name :

HUABIO

product type :

antibody

product name :

Integrin beta 1

catalog :

EM1901-77

quantity :

100μl

price :

360.00 USD

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

B10-A5

reactivity :

human, mouse

application :

western blot, flow cytometry, immunohistochemistry - paraffin section

more info or order :

HUABIO product webpage

image

image 1 :

Western blot analysis of Integrin beta 1 on different lysates with Mouse anti-Integrin beta 1 antibody (EM1901-77) at 1/2,000 dilution. Lane 1: HeLa cell lysate Lane 2: A549 cell lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 88 kDa Observed band size: 120-140 kDa Exposure time: 25 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (EM1901-77) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1/50,000 dilution was used for 1 hour at room temperature.

image 2 :

Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-Integrin beta 1 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the antibody (EM1901-77) at 1/200 dilution, for 30 minutes at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. Counter stained with hematoxylin and mounted with DPX

image 3 :

Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-Integrin beta 1 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the antibody (EM1901-77) at 1/50 dilution, for 30 minutes at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. Counter stained with hematoxylin and mounted with DPX

product information

SKU :

EM1901-77

Target name :

Integrin beta 1

Species reactivity :

Human,Mouse

Applications :

WB,IHC-P,FC

Conjugate :

Non-conjugated

Immunogen :

Synthetic peptide of the C-terminal Human Integrin beta 1.

Uniprot id :

P05556>SwissProt: P05556 Human;SwissProt: P09055 Mouse

Host :

Mouse

Clone number :

B10-A5

Isotype :

IgM

Size :

100μl

List Price :

360.00 USD

Storage Buffer :

1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.

Form :

Liquid

Storage Instruction :

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.

Purity :

Protein A affinity purified.

Product type :

Mouse monoclonal Antibody

Positive control :

HeLa cell lysate, A549 cell lysate, human tonsil tissue, human kidney tissue, L929.

Molecular wt :

Predicted band size: 88 kDa

Subcellular location :

Cell Membrane, Cell projection, Cleavage furrow.

Concentration :

2 mg/mL.

Recommended dilutions :

WB: 1:1000-1:5000 ;IHC-P: 1:50-1:200 ;FC: 1:50-1:100

Pic img4 :

https://storage.huabio.cn/huabio/productImg/EM1901-77_4.jpg

Pic legend4 :

Flow cytometric analysis of Integrin beta 1 was done on L929 cells. The cells were fixed, permeabilized and stained with the primary antibody (EM1901-77, 1/100) (red). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488 Goat anti-Mouse IgG Secondary antibody at 1/500 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; black).

more info or order :

HUABIO product webpage