product summary
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company name :
HUABIO
product type :
antibody
product name :
Annexin A3
catalog :
EM1901-73
quantity :
100μl
price :
360.00 USD
clonality :
monoclonal
host :
mouse
conjugate :
nonconjugated
clone name :
A1F7
reactivity :
human, mouse, rat
application :
western blot, flow cytometry, immunohistochemistry - paraffin section
more info or order :
image
image 1 :
Western blot analysis of Annexin A3 on different lysates with Mouse anti-Annexin A3 antibody (EM1901-73) at 1/2,000 dilution.
Lane 1: A431 cell lysate (20 µg/Lane)
Lane 2: HEK-293 cell lysate (20 µg/Lane)
Lane 3: HepG2 cell lysate (20 µg/Lane)
Lane 4: HeLa cell lysate (20 µg/Lane)
Lane 5: HT-29 cell lysate (20 µg/Lane)
Lane 6: A375 cell lysate (20 µg/Lane)
Lane 7: MCF7 cell lysate (20 µg/Lane)
Lane 8: Human lung tissue lysate (40 µg/Lane)
Lane 9: Mouse lung tissue lysate (40 µg/Lane)
Lane 10: Mouse testis tissue lysate (40 µg/Lane)
Lane 11: Rat testis tissue lysate (40 µg/Lane)
Predicted band size: 36 kDa
Observed band size: 33/36 kDa
Exposure time: 9 seconds;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (EM1901-73) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1/50,000 dilution was used for 1 hour at room temperature.
image 2 :
ICC staining of Annexin A3 in HT-29 cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (EM1901-73, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 Goat anti-Mouse IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).
image 3 :
ICC staining of Annexin A3 in SiHa cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (EM1901-73, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 Goat anti-Mouse IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).
product information
SKU :
EM1901-73
Target name :
Annexin A3
Species reactivity :
Human,Mouse,Rat
Applications :
WB,IF-Cell,IHC-P,FC
Conjugate :
Non-conjugated
Immunogen :
Recombinant protein within Human Annexin A3 aa 1-323 / 323.
Uniprot id :
P12429>SwissProt: P12429 Human;SwissProt: O35639 Mouse;SwissProt: P14669 Rat
Host :
Mouse
Clone number :
A1F7
Isotype :
IgG1
Size :
100μl
List Price :
360.00 USD
Storage Buffer :
1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
Form :
Liquid
Storage Instruction :
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
Purity :
Protein G affinity purified.
Product type :
Mouse monoclonal Antibody
Positive control :
A431 cell lysate, HEK-293 cell lysate, HepG2 cell lysate, HeLa cell lysate, HT-29 cell lysate, A375 cell lysate, MCF7 cell lysate, human lung tissue lysate, mouse lung tissue lysate, mouse testis tissue lysate, rat testis tissue lysate, HT-29, SiHa, human tonsil tissue, human kidney tissue, human pancreas tissue, mouse lung tissue, A431.
Molecular wt :
Predicted band size: 36 kDa
Subcellular location :
Cytosol, extracellular exosome, plasma membrane, axon, cytoplasm, dendrite, membrane, neuronal cell body, phagocytic vesicle membrane, specific granule.
Concentration :
2 mg/mL.
Recommended dilutions :
WB: 1:2,000
;IF-Cell:1:50-1:100
;IHC-P:1:50-1:500
;FC:1:50-1:100
Pic img4 :
https://storage.huabio.cn/huabio/productImg/EM1901-73_4.jpg
Pic legend4 :
Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-Annexin A3 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (EM1901-73, 1/400) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Pic img5 :
https://storage.huabio.cn/huabio/productImg/EM1901-73_5.jpg
Pic legend5 :
Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-Annexin A3 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (EM1901-73, 1/400) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Pic img6 :
https://storage.huabio.cn/huabio/productImg/EM1901-73_6.jpg
Pic legend6 :
Immunohistochemical analysis of paraffin-embedded human pancreas tissue using anti-Annexin A3 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (EM1901-73, 1/400) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Pic img7 :
https://storage.huabio.cn/huabio/productImg/EM1901-73_7.jpg
Pic legend7 :
Immunohistochemical analysis of paraffin-embedded mouse lung tissue using anti-Annexin A3 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (EM1901-73, 1/400) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Pic img8 :
https://storage.huabio.cn/huabio/productImg/EM1901-73_8.jpg
Pic legend8 :
Flow cytometric analysis of Annexin A3 was done on A431 cells. The cells were fixed, permeabilized and stained with the primary antibody (EM1901-73, 1/50) (red). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated Goat anti-Mouse IgG Secondary antibody at 1/1,000 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; black).
more info or order :
company information
HUABIO
Founded in 2007, HUABIO is dedicated to developing high-quality antibodies that advance innovation. We are passionate about the accuracy, efficiency, and consistency of our products. That is why we have invested in new production platforms, like recombinant rabbit monoclonals, alpaca nanobodies, and adopted aggressive QA standards to deliver cutting-edge antibodies with uncompromised quality.
We hope to see you at your next discovery!
We hope to see you at your next discovery!
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