CD43 | HUABIO EM1901-71 product information

product summary

company name :

HUABIO

product type :

antibody

product name :

CD43

catalog :

EM1901-71

quantity :

100μl

price :

360.00 USD

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

A2F9

reactivity :

human

application :

western blot, flow cytometry, immunohistochemistry - paraffin section

more info or order :

HUABIO product webpage

image

image 1 :

Western blot analysis of CD43 on different lysates with Mouse anti-CD43 antibody (EM1901-71) at 1/1,000 dilution. Lane 1: K-562 cell lysate Lane 2: HL-60 cell lysate Lane 3: THP-1 cell lysate Lane 4: U-937 cell lysate Lane 5: HT-29 cell lysate (negative) Lysates/proteins at 20 µg/Lane. Predicted band size: 40 kDa Observed band size: 100-130 kDa Exposure time: 1 minute 2 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (EM1901-71) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1/50,000 dilution was used for 1 hour at room temperature.

image 2 :

Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-CD43 antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (EM1901-71, 1/200) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

image 3 :

Flow cytometric analysis of CD43 was done on HL-60 cells. The cells were fixed, permeabilized and stained with the primary antibody (EM1901-71, 1/50) (red). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated Goat anti-Mouse IgG Secondary antibody at 1/1,000 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; black).

product information

SKU :

EM1901-71

Target name :

CD43

Species reactivity :

Human

Applications :

WB,IHC-P,FC

Conjugate :

Non-conjugated

Immunogen :

Synthetic peptide within C-terminal human CD43.

Uniprot id :

P16150>SwissProt: P16150 Human

Host :

Mouse

Clone number :

A2F9

Isotype :

IgG1

Size :

100μl

List Price :

360.00 USD

Storage Buffer :

1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.

Form :

Liquid

Storage Instruction :

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.

Purity :

Protein G affinity purified.

Product type :

Mouse monoclonal Antibody

Positive control :

K-562 cell lysate, HL-60 cell lysate, THP-1 cell lysate, U-937 cell lysate, human tonsil tissue, HL-60.

Molecular wt :

Predicted band size: 40 kDa

Subcellular location :

Membrane, microvillus, uropodium; Nucleus, PML body.

Concentration :

2 mg/mL.

Recommended dilutions :

WB:1:1,000 ;IHC-P:1:50-1:200 ;FC:1:50-1:100

Advanced Validation :

Relative expression (RE)

more info or order :

HUABIO product webpage