EGFR | HUABIO EM1901-67 product information

product summary

company name :

HUABIO

product type :

antibody

product name :

EGFR

catalog :

EM1901-67

quantity :

100μl

price :

360.00 USD

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

7-F2-F8

reactivity :

human

application :

western blot, flow cytometry, immunohistochemistry - paraffin section

more info or order :

HUABIO product webpage

image

image 1 :

Western blot analysis of EGFR on different lysates with Mouse anti-EGFR antibody (EM1901-67) at 1/2,000 dilution and competitor's antibody at 1/2,000 dilution. Lane 1: HeLa cell lysate Lane 2: A431 cell lysate Lane 3: MDA-MB-468 cell lysate Lane 4: MCF7 cell lysate (low expression) Lysates/proteins at 15 µg/Lane. Predicted band size: 134 kDa Observed band size: 150 kDa Exposure time: Lane 1-4 (left): 5 seconds; Lane 1-4 (right): 21 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (EM1901-67) at 1/2,000 dilution and competitor's antibody at 1/2,000 dilution were used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1/50,000 dilution was used for 1 hour at room temperature.

image 2 :

All lanes: Western blot analysis of EGFR with anti-EGFR antibody [7-F2-F8] (EM1901-67) at 1/500 dilution. Lane 1: Wild-type MC3T3 whole cell lysate. Lane 2: EGFR knockout MC3T3 whole cell lysate. EM1901-67 was shown to specifically react with EGFR in Wild-type MC3T3 cells. No band was observed when EGFR knockout sample was tested. Wild-type and EGFR knockout samples were subjected to SDS-PAGE. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM in TBST for 1 hour at room temperature. The primary Anti-EGFR antibody (EM1901-67, 1/500) and Anti-Vinculin antibody (ET1705-94, 1/5,000) were used in 5% BSA at room temperature for 2 hours. Goat Anti-Mouse IgG HRP Secondary Antibody (HA1006) at 1:20,000 dilution was used for 1 hour at room temperature. Cell lysate was provided by Ubigene Biosciences (Ubigene Biosciences Co., Ltd., Guangzhou, China).

image 3 :

Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using anti-EGFR antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (EM1901-67, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

product information

SKU :

EM1901-67

Target name :

EGFR

Species reactivity :

Human,Monkey

Applications :

WB,IHC-P,FC

Conjugate :

Non-conjugated

Immunogen :

Recombinant protein within human EGFR aa 900-1150.

Uniprot id :

P00533>SwissProt: P00533 Human

Host :

Mouse

Clone number :

7-F2-F8

Isotype :

IgG2b

Size :

100μl

List Price :

360.00 USD

Storage Buffer :

1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.

Form :

Liquid

Storage Instruction :

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.

Purity :

Protein A affinity purified.

Product type :

Mouse monoclonal Antibody

Positive control :

HeLa cell lysate, A431 cell lysate, MDA-MB-468 cell lysate, Wild-type MC3T3 whole cell lysate, human breast carcinoma tissue, human placenta tissue, A431.

Molecular wt :

Predicted band size: 134 kDa

Subcellular location :

Golgi apparatus membrane, nucleus membrane, nucleus, cell membrane, endosome, endosome membrane, endoplasmic reticulum membrane, cytoplasm.

Concentration :

2 mg/mL.

Recommended dilutions :

WB: 1:2,000 ;IHC-P: 1:50-1:200 ;FC: 1:1,000

Advanced Validation :

Relative expression (RE),Knockout (KO)

Pic img4 :

https://storage.huabio.cn/huabio/productImg/EM1901-67_4.jpg

Pic legend4 :

Immunohistochemical analysis of paraffin-embedded human placenta tissue using anti-EGFR antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (EM1901-67, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Pic img5 :

https://storage.huabio.cn/huabio/productImg/EM1901-67_5.jpg

Pic legend5 :

Flow cytometric analysis of A431 cells labeling EGFR. Cells were fixed and permeabilized. Then stained with the primary antibody (EM1901-67, 1μg/mL) (red) compared with Mouse IgG1 Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Mouse IgG Secondary antibody (HA1125) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).

more info or order :

HUABIO product webpage