UBA3 | HUABIO EM1901-65 product information

product summary

company name :

HUABIO

product type :

antibody

product name :

UBA3

catalog :

EM1901-65

quantity :

100μl

price :

360.00 USD

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

1C10-5-5

reactivity :

human, mouse, rat

application :

western blot, flow cytometry, immunohistochemistry - paraffin section

more info or order :

HUABIO product webpage

image

image 1 :

Western blot analysis of UBA3 on different lysates with Mouse anti-UBA3 antibody (EM1901-65) at 1/1,000 dilution. Lane 1: HeLa cell lysate (20 µg/Lane) Lane 2: 293T cell lysate (20 µg/Lane) Lane 3: HepG2 cell lysate (20 µg/Lane) Lane 4: K-562 cell lysate (20 µg/Lane) Lane 5: HL-60 cell lysate (20 µg/Lane) Lane 6: Jurkat cell lysate (20 µg/Lane) Lane 7: Mouse brain tissue lysate (40 µg/Lane) Lane 8: Rat brain tissue lysate (40 µg/Lane) Predicted band size: 52 kDa Observed band size: 52 kDa Exposure time: 3 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (EM1901-65) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1/50,000 dilution was used for 1 hour at room temperature.

image 2 :

Immunohistochemical analysis of paraffin-embedded human brain tissue with Mouse anti-UBA3 antibody (EM1901-65) at 1/2,000 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (EM1901-65) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

image 3 :

Immunohistochemical analysis of paraffin-embedded mouse brain tissue with Mouse anti-UBA3 antibody (EM1901-65) at 1/2,000 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (EM1901-65) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

product information

SKU :

EM1901-65

Target name :

UBA3

Species reactivity :

Human,Mouse,Rat

Applications :

WB,IHC-P,FC

Conjugate :

Non-conjugated

Immunogen :

Recombinant protein within Human UBA3 aa 220-424 / 463.

Uniprot id :

Q8TBC4>SwissProt: Q8TBC4 Human;SwissProt: Q8C878 Mouse;SwissProt: Q99MI7 Rat

Host :

Mouse

Clone number :

1C10-5-5

Isotype :

IgG1

Size :

100μl

List Price :

360.00 USD

Storage Buffer :

1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.

Form :

Liquid

Storage Instruction :

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.

Purity :

Protein A affinity purified.

Product type :

Mouse monoclonal Antibody

Positive control :

HeLa cell lysate, 293T cell lysate, HepG2 cell lysate, K-562 cell lysate, HL-60 cell lysate, Jurkat cell lysate, mouse brain tissue lysate, rat brain tissue lysate, human brain tissue, mouse brain tissue, rat brain tissue, human lung tissue, human lung carcinoma tissue, human skin tissue, human breast carcinoma tissue, human esophagus tissue, SiHa.

Molecular wt :

Predicted band size: 52 kDa

Subcellular location :

Cytosol, nucleus, cytoplasm.

Concentration :

2 mg/mL.

Recommended dilutions :

WB: 1:500-1:2,000 ;IHC-P: 1:200-1:2,000 ;FC: 1:50-1:100

Pic img4 :

https://storage.huabio.cn/huabio/productImg/EM1901-65_4.jpg

Pic legend4 :

Immunohistochemical analysis of paraffin-embedded rat brain tissue with Mouse anti-UBA3 antibody (EM1901-65) at 1/2,000 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (EM1901-65) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Pic img5 :

https://storage.huabio.cn/huabio/productImg/EM1901-65_5.jpg

Pic legend5 :

Immunohistochemical analysis of paraffin-embedded human lung tissue using anti-UBA3 antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (EM1901-65, 1/400) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Pic img6 :

https://storage.huabio.cn/huabio/productImg/EM1901-65_6.jpg

Pic legend6 :

Immunohistochemical analysis of paraffin-embedded human lung carcinoma tissue using anti-UBA3 antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (EM1901-65, 1/400) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Pic img7 :

https://storage.huabio.cn/huabio/productImg/EM1901-65_7.jpg

Pic legend7 :

Immunohistochemical analysis of paraffin-embedded human skin tissue using anti-UBA3 antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (EM1901-65, 1/400) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Pic img8 :

https://storage.huabio.cn/huabio/productImg/EM1901-65_8.jpg

Pic legend8 :

Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using anti-UBA3 antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (EM1901-65, 1/200) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Pic img9 :

https://storage.huabio.cn/huabio/productImg/EM1901-65_9.jpg

Pic legend9 :

Immunohistochemical analysis of paraffin-embedded human esophagus tissue using anti-UBA3 antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (EM1901-65, 1/200) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Pic img10 :

https://storage.huabio.cn/huabio/productImg/EM1901-65_10.jpg

Pic legend10 :

Flow cytometric analysis of UBA3 was done on SiHa cells. The cells were fixed, permeabilized and stained with the primary antibody (EM1901-65, 1/50) (red). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated Goat anti-Mouse IgG Secondary antibody at 1/1,000 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; black).

more info or order :

HUABIO product webpage